{"id":"W4399774982","doi":"10.1002/edn3.578","title":"Systematic evaluation of the influence of Taq enzyme choice and amplification conditions on targeted environmental DNA assay performance and detection in field samples","year":2024,"lang":"en","type":"article","venue":"Environmental DNA","topic":"Environmental DNA in Biodiversity Studies","field":"Environmental Science","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Victoria","funders":"Natural Sciences and Engineering Research Council of Canada; Genome British Columbia; Genome Canada","keywords":"DNA; Computational biology; Chromatography; Molecular biology; Environmental science; Chemistry; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.02903519,0.002039725,0.001518918,0.001580521,0.00104093,0.001376389,0.001441283,0.001310752,0.0009828802],"category_scores_gemma":[0.03332693,0.001133361,0.001030116,0.001664274,0.001726714,0.0008669024,0.001544173,0.00106316,0.0007062168],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007827784,"about_ca_system_score_gemma":0.001630051,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002542194,"about_ca_topic_score_gemma":0.006680784,"domain_scores_codex":[0.9701393,0.01136976,0.004318964,0.004569324,0.008652455,0.0009502682],"domain_scores_gemma":[0.9717753,0.01370621,0.003152208,0.00249966,0.008570943,0.0002955718],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00022528,0.0003268232,0.006169949,0.0008356889,0.0001258429,0.00009240706,0.0006376664,0.001709327,0.9753117,0.0001612575,0.0002445833,0.01415948],"study_design_scores_gemma":[0.00002420943,0.002123582,0.009928021,0.000144756,0.0002649395,0.0002181329,0.0002287379,0.003746135,0.9777734,0.0001348909,0.005336605,0.00007666276],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6701284,0.005655224,0.3141274,0.0003605161,0.0003385324,0.004193747,0.001420196,0.001025328,0.002750642],"genre_scores_gemma":[0.5212911,0.003922435,0.4609488,0.0005355913,0.00008082403,0.00618607,0.003669172,0.0008507522,0.002515137],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02903519,"threshold_uncertainty_score":0.1535546,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01205971243831512,"score_gpt":0.2188130740005834,"score_spread":0.2067533615622683,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}