{"id":"W4399983783","doi":"10.1007/978-1-0716-3946-7_17","title":"Single-Cell MTT: A Simple and Sensitive Assay for Determining the Viability and Metabolic Activity of Polyploid Giant Cancer Cells (PGCCs)","year":2024,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Cancer, Hypoxia, and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta; Alberta Biodiversity Monitoring Institute","funders":"","keywords":"Polyploid; Cancer cell; MTT assay; Viability assay; Cancer; Simple (philosophy); Biology; Chemistry; Cell; Botany; Biochemistry; Genetics; Ploidy; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001135528,0.002710845,0.001975847,0.002729986,0.0008841749,0.0009429016,0.002418746,0.001852171,0.002584801],"category_scores_gemma":[0.0008136457,0.000849486,0.0008719816,0.001982375,0.0006647865,0.001127903,0.001090495,0.003557644,0.003178749],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006321652,"about_ca_system_score_gemma":0.0007529186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00128042,"about_ca_topic_score_gemma":0.004165779,"domain_scores_codex":[0.9973025,0.0003343593,0.0002997158,0.0004634705,0.001410733,0.0001891777],"domain_scores_gemma":[0.9986278,0.0003094196,0.0002889156,0.0002561179,0.0003561105,0.0001616838],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007484006,0.00006303842,0.0003505197,0.000212845,0.00001904711,0.00006429289,0.0000795562,0.00007252025,0.9913573,0.0002739664,0.001042899,0.006389206],"study_design_scores_gemma":[0.0000114465,0.0001275411,0.001263549,0.00001537734,0.00004289239,0.0003230502,0.00001959318,0.0006308072,0.9914008,0.0001645849,0.005972833,0.00002751747],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1928948,0.01989834,0.7325301,0.001309581,0.001623817,0.001214192,0.01907922,0.01528955,0.0161604],"genre_scores_gemma":[0.326653,0.01637824,0.5667834,0.00075028,0.0005359187,0.003564064,0.02810214,0.002144746,0.05508824],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002729986,"threshold_uncertainty_score":0.008647084,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02026790759857489,"score_gpt":0.35130075658369,"score_spread":0.3310328489851151,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}