{"id":"W4400303479","doi":"10.1038/s42003-024-06422-z","title":"Structure and engineering of Brevibacillus laterosporus Cas9","year":2024,"lang":"en","type":"article","venue":"Communications Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Bio-oriented Technology Research Advancement Institution; Japan Society for the Promotion of Science; Cabinet Office, Government of Japan; Core Research for Evolutional Science and Technology; Japan Agency for Medical Research and Development","keywords":"Biology; Microbiology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001493726,0.0003851964,0.0002915193,0.0001618602,0.0003140566,0.0004555148,0.0005664779,0.000469059,0.0007110006],"category_scores_gemma":[0.0001781272,0.0002200683,0.0002327758,0.0002192774,0.0001993256,0.0001583741,0.0001706702,0.0006125207,0.0003624104],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008280926,"about_ca_system_score_gemma":0.0004772448,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003804787,"about_ca_topic_score_gemma":0.003156391,"domain_scores_codex":[0.9998962,0.00001589287,0.000009596734,0.00001865501,0.00004702132,0.00001264371],"domain_scores_gemma":[0.9999204,0.000009272401,0.00002403427,0.000009132407,0.00001302796,0.00002413275],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001382819,0.00009791677,0.0005540997,0.00007777231,0.00001452186,0.0003782475,0.00004643417,0.006629299,0.9875387,0.001421993,0.0002800235,0.002822723],"study_design_scores_gemma":[0.00008751633,0.0002042908,0.001660868,0.00001176234,0.00001879548,0.000365195,0.00006360418,0.02755144,0.9597511,0.0003255169,0.009934,0.00002592265],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9733781,0.0008122401,0.0209865,0.0003705392,0.00008335855,0.0001266175,0.001002378,0.0003402159,0.002900139],"genre_scores_gemma":[0.9688964,0.000521497,0.02576877,0.00003575001,0.000006474754,0.00004750407,0.001706452,0.00004947165,0.002967653],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003804787,"threshold_uncertainty_score":0.00756526,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009475952932286622,"score_gpt":0.3242820316779139,"score_spread":0.3148060787456273,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}