{"id":"W4400572453","doi":"10.3389/fmicb.2024.1381097","title":"Molecular cloning and functional analysis of a plastidial ω3 desaturase from Emiliania huxleyi","year":2024,"lang":"en","type":"article","venue":"Frontiers in Microbiology","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"Saskatchewan Research Council (Canada); University of Saskatchewan","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Emiliania huxleyi; Polyunsaturated fatty acid; Biochemistry; Docosahexaenoic acid; Biology; Heterologous expression; Linolenic acid; Arachidonic acid; Fatty acid desaturase; Linoleic acid; Chloroplast; Synechococcus; Fatty acid; Gene; Cyanobacteria; Enzyme; Genetics; Bacteria; Phytoplankton","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001280561,0.000427023,0.0003445054,0.0002830993,0.0001852303,0.0002779507,0.0002114698,0.0002669269,0.0004599243],"category_scores_gemma":[0.0001057815,0.0001245169,0.0004423528,0.0004043718,0.0001502741,0.0001748323,0.0002919476,0.0004288412,0.0003138875],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004105032,"about_ca_system_score_gemma":0.0002613298,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002817158,"about_ca_topic_score_gemma":0.001960293,"domain_scores_codex":[0.9999138,0.00000702895,0.00001073098,0.00002703522,0.00002250144,0.00001880966],"domain_scores_gemma":[0.9999124,0.00001724594,0.00002296519,0.000008793476,0.00001494965,0.00002361025],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000074526,0.00002486344,0.0005748391,0.00006573336,0.00000641187,0.00009036234,0.000034325,0.00007087828,0.9968101,0.00007741664,0.0000246128,0.002145886],"study_design_scores_gemma":[0.00004983867,0.0002334332,0.05918776,0.00002659695,0.00009565617,0.0008095199,0.0002053426,0.002540448,0.9253975,0.0001436945,0.01127824,0.00003194832],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.99005,0.00159074,0.00472546,0.00018486,0.00004153953,0.00005853573,0.001935435,0.00005538316,0.001358077],"genre_scores_gemma":[0.9656175,0.002312452,0.01099145,0.0001273672,0.00003209168,0.00006485649,0.01343921,0.0000431284,0.007371912],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002817158,"threshold_uncertainty_score":0.005601525,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003772794983411316,"score_gpt":0.1962513089970053,"score_spread":0.192478514013594,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}