{"id":"W4400590870","doi":"10.1093/nar/gkae604","title":"Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET","year":2024,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Key Research and Development Program of China; National Natural Science Foundation of China; Ministry of Agriculture - Saskatchewan; Beijing Frontier Research Center for Biological Structure, Tsinghua University","keywords":"Biology; Cas9; CRISPR; Cleavage (geology); DNA; Förster resonance energy transfer; Genome editing; Computational biology; Effector; Biophysics; Genome; Nuclease; Genetics; Cell biology; Gene; Fluorescence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007476031,0.0001062331,0.0001269768,0.0001566616,0.00004254759,0.00003956513,0.0002182775,0.0001565174,0.00004692486],"category_scores_gemma":[0.0002190612,0.0001095955,0.00005265265,0.0003098044,0.0001072391,0.000007335981,0.0001598216,0.0002795462,0.00001628866],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006659475,"about_ca_system_score_gemma":0.00009235366,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004200973,"about_ca_topic_score_gemma":0.00006748156,"domain_scores_codex":[0.9986575,0.00007084663,0.0002950693,0.0002471569,0.0003875996,0.0003418613],"domain_scores_gemma":[0.9995098,0.00006358969,0.00002235903,0.0002317871,0.00009611133,0.00007638137],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002644155,0.00003583179,0.000989173,0.0001790725,0.00002526026,0.00001325345,0.0002486514,0.00009007109,0.9869463,0.0003785026,0.006318731,0.004748647],"study_design_scores_gemma":[0.0006563246,0.0004838057,0.00190365,0.0001568877,0.000007990766,0.00003138307,0.0007387688,0.04408674,0.937631,0.0002511584,0.013742,0.000310333],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9884788,0.001337175,0.002512976,0.0002989022,0.00009833976,0.0001791566,0.0001201807,0.00001687255,0.00695755],"genre_scores_gemma":[0.9984139,0.0001291311,0.0004694195,0.00002352066,0.00009437279,0.00001418228,0.0002941913,0.00002841343,0.0005329195],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0493154,"threshold_uncertainty_score":0.4469176,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01806548383698804,"score_gpt":0.3501703029249579,"score_spread":0.3321048190879699,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}