{"id":"W4400590870","doi":"10.1093/nar/gkae604","title":"Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET","year":2024,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Key Research and Development Program of China; National Natural Science Foundation of China; Ministry of Agriculture - Saskatchewan; Beijing Frontier Research Center for Biological Structure, Tsinghua University","keywords":"Biology; Cas9; CRISPR; Cleavage (geology); DNA; Förster resonance energy transfer; Genome editing; Computational biology; Effector; Biophysics; Genome; Nuclease; Genetics; Cell biology; Gene; Fluorescence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002840065,0.0002159398,0.0002741097,0.00009065704,0.0001742181,0.0004165832,0.000421949,0.0004189101,0.00082509],"category_scores_gemma":[0.0004689166,0.0001593173,0.0001613401,0.0001168761,0.0002901022,0.0006288313,0.0002533747,0.0007455116,0.0002573171],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004890336,"about_ca_system_score_gemma":0.0002299616,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007396812,"about_ca_topic_score_gemma":0.0007609081,"domain_scores_codex":[0.9998606,0.00001420937,0.000007210449,0.00005068685,0.00003545711,0.00003192203],"domain_scores_gemma":[0.9998571,0.00005946336,0.00002907231,0.00001665635,0.000018981,0.0000187214],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000739836,0.00003709575,0.0003631622,0.00003989069,0.000006898361,0.00005170934,0.00006145881,0.0008603418,0.9951226,0.0006734452,0.00009391049,0.002615471],"study_design_scores_gemma":[0.00001133793,0.00008525314,0.002226521,0.00000508951,0.000006431866,0.00008738178,0.00006418389,0.02071841,0.9755157,0.0002445964,0.001021416,0.00001383776],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9894883,0.0003199466,0.0083808,0.0001090172,0.00001500446,0.00001708263,0.0001322191,0.00007393882,0.001463674],"genre_scores_gemma":[0.9955414,0.0001971508,0.003120538,0.00002865917,0.000002323051,0.00002461192,0.0001156369,0.00001643097,0.0009531073],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00082509,"threshold_uncertainty_score":0.003548145,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01806548383698804,"score_gpt":0.3501703029249579,"score_spread":0.3321048190879699,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}