{"id":"W4400592793","doi":"10.1039/d4fd00017j","title":"Indigo production identifies hotspots in cytochrome P450 BM3 for diversifying aromatic hydroxylation","year":2024,"lang":"en","type":"article","venue":"Faraday Discussions","topic":"Metabolomics and Mass Spectrometry Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; PROTEO; Centre in Green Chemistry and Catalysis","funders":"Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Indigo; Hydroxylation; Cytochrome P450; Chemistry; Indigo carmine; Phenotype; Biochemistry; Enzyme; Gene; Art","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001715868,0.0003751711,0.0003086063,0.0005437327,0.0001754136,0.0004222156,0.0003031418,0.0003856805,0.001837251],"category_scores_gemma":[0.0003128994,0.0002249595,0.0002818658,0.000376373,0.0002279505,0.0001845002,0.0004294641,0.0005191277,0.0007639782],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002737567,"about_ca_system_score_gemma":0.0001346369,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000625617,"about_ca_topic_score_gemma":0.00142017,"domain_scores_codex":[0.9998469,0.00001872171,0.000008749136,0.00003608842,0.00005850437,0.00003096731],"domain_scores_gemma":[0.9998047,0.00004640433,0.00005315657,0.00003776641,0.00001747223,0.00004044455],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001565523,0.000008240951,0.0003337823,0.000003698144,0.000001750123,0.00002058668,0.000004863548,0.00003172112,0.9988186,0.00002614724,0.00001145055,0.0007235582],"study_design_scores_gemma":[0.000003622565,0.00006905135,0.007834697,0.000002279982,0.000009795001,0.0003583628,0.00002303594,0.0007685678,0.9902061,0.00004767727,0.000672761,0.000003998854],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9807768,0.0002949414,0.01650989,0.00009088374,0.0000124062,0.00003135432,0.0005083221,0.0003166961,0.001458699],"genre_scores_gemma":[0.9849354,0.0001916127,0.01099146,0.00005332546,0.000003776935,0.00002764542,0.001355964,0.000095193,0.002345665],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001837251,"threshold_uncertainty_score":0.006146252,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01505969858783802,"score_gpt":0.2848008623983018,"score_spread":0.2697411638104638,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}