{"id":"W4400613718","doi":"10.1186/s44316-024-00007-w","title":"Inexpensive and colorimetric RNA detection at ambient temperature with a cell-free protein synthesis platform","year":2024,"lang":"en","type":"article","venue":"Biotechnology for Sustainable Materials","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"","keywords":"RNA; lac operon; Gene expression; Complementation; In vitro; Translation (biology); Cell; Transcription (linguistics); Gene; Chemistry; Computational biology; Messenger RNA; Molecular biology; Cell biology; Biology; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001340327,0.001743803,0.0007373667,0.0009931107,0.0002978778,0.000896179,0.001262743,0.001369395,0.006005616],"category_scores_gemma":[0.001042007,0.0006753236,0.0008059442,0.000723031,0.000500348,0.0007486252,0.0006287106,0.002167402,0.005667492],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005513831,"about_ca_system_score_gemma":0.0004209599,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002074253,"about_ca_topic_score_gemma":0.0005290343,"domain_scores_codex":[0.9982005,0.0002991163,0.0000820701,0.0004640188,0.0007973392,0.00015683],"domain_scores_gemma":[0.9993085,0.000246443,0.0001698468,0.00007725223,0.0001527712,0.00004524487],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003103494,0.0000214479,0.0000528934,0.0002870669,0.00001293364,0.00004780581,0.00001438733,0.0002984643,0.9922218,0.0005690987,0.0008826302,0.00556049],"study_design_scores_gemma":[0.00001123176,0.0001455847,0.0001924245,0.00001744144,0.00001382343,0.0001383243,0.00001354883,0.001379015,0.9886237,0.0002445052,0.009193474,0.00002694711],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1115668,0.007215513,0.8509858,0.001403282,0.001282534,0.0007291111,0.005074276,0.006473321,0.01526934],"genre_scores_gemma":[0.4177678,0.007714753,0.5448062,0.001078069,0.0003878392,0.001466217,0.007054838,0.0006148004,0.01910951],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.006005616,"threshold_uncertainty_score":0.02009076,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003307357686050629,"score_gpt":0.2207993886946934,"score_spread":0.2174920310086428,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}