{"id":"W4400676936","doi":"10.3390/antib13030057","title":"Humanization of Pan-HLA-DR mAb 44H10 Hinges on Critical Residues in the Antibody Framework","year":2024,"lang":"en","type":"article","venue":"Antibodies","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada Research Chairs; Hospital for Sick Children; University of Toronto","funders":"Canadian Light Source; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; National Cancer Institute; Canada Research Chairs; National Institutes of Health; Hospital for Sick Children; Office of Science; National Institute of General Medical Sciences; Canadian Institute for Advanced Research; Argonne National Laboratory; U.S. Department of Energy","keywords":"Paratope; Monoclonal antibody; Antibody; Antigen; Immunogenicity; Complementarity determining region; Human leukocyte antigen; Major histocompatibility complex; Computational biology; Biology; Humanized antibody; Immunology; Cell biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004972985,0.0001922972,0.0003396831,0.0002877992,0.0001316059,0.00008163584,0.0002088949,0.0001346549,0.0002384575],"category_scores_gemma":[0.0007734736,0.0001165509,0.0001311569,0.0004289736,0.0003849802,0.0001100532,0.00007493655,0.0005883268,0.0001259507],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002367329,"about_ca_system_score_gemma":0.00007509084,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002654738,"about_ca_topic_score_gemma":0.00005255882,"domain_scores_codex":[0.9981708,0.0001425352,0.0003528398,0.0003226271,0.0006548679,0.0003563346],"domain_scores_gemma":[0.9979936,0.001490536,0.00002840989,0.0003105078,0.0001257543,0.00005122402],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"theoretical_or_conceptual","study_design_gemma":"observational","study_design_scores_codex":[0.0005359104,0.0005861532,0.1408422,0.002378747,0.0001694732,0.0009773065,0.005846061,0.000005510242,0.01474468,0.8143571,0.01284335,0.006713525],"study_design_scores_gemma":[0.000616011,0.002243706,0.8296942,0.007554028,0.0002144436,0.0002296055,0.003337449,0.0006991404,0.0291214,0.09499153,0.03072769,0.0005708068],"study_design_candidate":"observational","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9475863,0.01099883,0.0001712693,0.02743377,0.0003538313,0.0003316492,0.00003957188,0.00008900264,0.01299575],"genre_scores_gemma":[0.9942878,0.00274871,0.0005947268,0.0007605012,0.0004324577,0.000008900334,0.00006307576,0.00002591149,0.001077884],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.7193655,"threshold_uncertainty_score":0.4752806,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04654255567493678,"score_gpt":0.4254096671673216,"score_spread":0.3788671114923849,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}