{"id":"W4400869843","doi":"10.1038/s41587-024-02324-x","title":"Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases","year":2024,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":65,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"NIH Office of the Director; National Cancer Institute; National Center of Competence in Research Quantum Science and Technology; National Heart, Lung, and Blood Institute; Dana-Farber Cancer Institute; National Human Genome Research Institute; National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; Massachusetts General Hospital; Natural Sciences and Engineering Research Council of Canada; Friedreich's Ataxia Research Alliance; National Institutes of Health; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; European Molecular Biology Organization; National Science Foundation","keywords":"Genome editing; DNA polymerase; Polymerase; DNA; Endonuclease; Computational biology; Biology; Genome; Genetics; Computer science; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001134281,0.0001974121,0.0001572612,0.0001568123,0.0001128049,0.0000787352,0.0001452028,0.0008629177,0.00001034381],"category_scores_gemma":[0.0001039766,0.0001940675,0.00006286166,0.0002042617,0.0001272908,0.000006236039,0.0002030814,0.0004315398,0.000002541159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001420134,"about_ca_system_score_gemma":0.00003674071,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001688679,"about_ca_topic_score_gemma":0.00001191049,"domain_scores_codex":[0.9988337,0.00001485758,0.0001810393,0.0004981864,0.00008088004,0.0003913437],"domain_scores_gemma":[0.9996224,0.00001886768,0.00003133061,0.000242957,0.00002678784,0.00005763348],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000006828999,0.00001196951,0.0001634197,0.00009922188,0.0000538252,0.00003160014,0.0000121264,0.00003436216,0.977376,0.0008297446,0.0001223897,0.02125847],"study_design_scores_gemma":[0.0001509745,0.0001242041,0.0001072624,0.00006826417,0.00003596162,0.0003315678,0.0001557833,0.000880513,0.8641759,0.00007541283,0.1336572,0.0002370689],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9006718,0.09467657,0.003078427,0.000578918,0.0003780158,0.0001703486,0.00002988465,0.0002469925,0.0001690003],"genre_scores_gemma":[0.9933887,0.0009136156,0.004610101,0.0001197295,0.0007681256,0.00001041263,0.00004652224,0.00004507918,0.00009774605],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1335348,"threshold_uncertainty_score":0.7913843,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004968588236900854,"score_gpt":0.285832384237509,"score_spread":0.2808637960006081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}