{"id":"W4400869843","doi":"10.1038/s41587-024-02324-x","title":"Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases","year":2024,"lang":"en","type":"article","venue":"Nature Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":65,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"NIH Office of the Director; National Cancer Institute; National Center of Competence in Research Quantum Science and Technology; National Heart, Lung, and Blood Institute; Dana-Farber Cancer Institute; National Human Genome Research Institute; National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; Massachusetts General Hospital; Natural Sciences and Engineering Research Council of Canada; Friedreich's Ataxia Research Alliance; National Institutes of Health; Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; European Molecular Biology Organization; National Science Foundation","keywords":"Genome editing; DNA polymerase; Polymerase; DNA; Endonuclease; Computational biology; Biology; Genome; Genetics; Computer science; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004532127,0.0005111536,0.0005237659,0.0003916553,0.0003122182,0.001125995,0.0009777658,0.0007373987,0.005681668],"category_scores_gemma":[0.0007500609,0.0004978302,0.0003915613,0.000313924,0.0005499363,0.0009026802,0.0009327555,0.001378528,0.001888274],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003580448,"about_ca_system_score_gemma":0.0002745628,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003829244,"about_ca_topic_score_gemma":0.001047008,"domain_scores_codex":[0.9994619,0.00003616401,0.00003172261,0.0001465521,0.0002427471,0.00008093425],"domain_scores_gemma":[0.9993621,0.0002681378,0.0001153433,0.0001271624,0.00005051288,0.00007686826],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001042067,0.0000420058,0.0002312135,0.00008670334,0.00001062047,0.0001925187,0.00004704113,0.0003078768,0.9757426,0.003479537,0.001345933,0.01840969],"study_design_scores_gemma":[0.00000974197,0.00003465389,0.0003011895,0.000004993309,0.000005913173,0.0001333898,0.000009264506,0.001756352,0.988793,0.0004792247,0.008454306,0.00001801217],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5379571,0.002106174,0.4033002,0.0008464637,0.000892558,0.0002326069,0.002042112,0.01995243,0.03267033],"genre_scores_gemma":[0.8642166,0.001413446,0.09593231,0.0003353427,0.0001195217,0.0001682165,0.001744711,0.001677698,0.03439224],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005681668,"threshold_uncertainty_score":0.01900709,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004968588236900854,"score_gpt":0.285832384237509,"score_spread":0.2808637960006081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}