{"id":"W4401055955","doi":"10.1016/j.xpro.2024.103208","title":"A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells","year":2024,"lang":"en","type":"article","venue":"STAR Protocols","topic":"interferon and immune responses","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"National Institutes of Health; Institute of Infection and Immunity; Canadian Institutes of Health Research; Dalhousie University; Natural Sciences and Engineering Research Council of Canada; Dalhousie Medical Research Foundation","keywords":"Diacylglycerol kinase; Protein kinase C; Cell biology; Bone marrow; Chemistry; Kinase; Biology; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008482484,0.0002467025,0.0003760554,0.0001769556,0.000116727,0.0000452498,0.0003262534,0.0001800831,0.0003268943],"category_scores_gemma":[0.000190804,0.0001690911,0.0001808659,0.0001962625,0.0002240014,0.0001370347,0.0001317365,0.0004221149,0.00006487447],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008641599,"about_ca_system_score_gemma":0.0002737149,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000120277,"about_ca_topic_score_gemma":0.00008713835,"domain_scores_codex":[0.9981426,0.0005138177,0.0004616165,0.0003592998,0.00006592817,0.0004567418],"domain_scores_gemma":[0.9991021,0.0002438905,0.0001153806,0.0004246224,0.00009608168,0.00001798277],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.004294208,0.0002893932,0.00001362896,0.001496522,0.00006724295,0.00001346955,0.0004275989,0.000002048113,0.9875365,0.000470859,0.0004143531,0.004974205],"study_design_scores_gemma":[0.002609023,0.0008603936,0.0001696786,0.001524068,0.000009107019,0.00001509391,0.0000862684,0.00003783741,0.9024326,0.0002605085,0.0918193,0.0001761142],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.1151484,0.00006002804,0.0002506157,0.0002633511,0.00008009301,0.8838112,0.00006450944,0.00008554485,0.0002362336],"genre_scores_gemma":[0.3273605,1.839544e-7,0.0001022735,0.0000161416,0.00001610961,0.671343,0.000003243149,0.00002572752,0.001132851],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.2124683,"threshold_uncertainty_score":0.6895334,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04246265814328685,"score_gpt":0.3289222047801749,"score_spread":0.2864595466368881,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}