{"id":"W4401277257","doi":"10.3389/fsybi.2024.1419557","title":"Global incorporation of meta-fluorotyrosine or meta-fluorophenylalanine into 1,2-catechol dioxygenase modulates the binding affinities of substrates","year":2024,"lang":"en","type":"article","venue":"Frontiers in Synthetic Biology","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan; University of Ottawa; National Research Council Canada; University of Manitoba","funders":"National Research Council Canada; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; University of Ottawa","keywords":"Affinities; Binding affinities; Catechol; Stereochemistry; Chemistry; Dioxygenase; Genetics; Biology; Biochemistry; Enzyme; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001133821,0.0002225748,0.0001861177,0.0000654539,0.00007590144,0.0002115234,0.0001967159,0.0002070862,0.0005244036],"category_scores_gemma":[0.0001289668,0.00009771605,0.0001567478,0.00006669515,0.0001917563,0.0001359041,0.0001442911,0.0002906575,0.0001092818],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002578584,"about_ca_system_score_gemma":0.0001701822,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001362348,"about_ca_topic_score_gemma":0.001566201,"domain_scores_codex":[0.9999098,0.00001128772,0.000006761698,0.00002439569,0.00002189307,0.00002578392],"domain_scores_gemma":[0.9999169,0.00002013881,0.00002504887,0.00001144199,0.00000910771,0.00001744462],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005881984,0.00001066344,0.0001921357,0.000009656826,0.000002727896,0.00001391445,0.000007450853,0.00008707662,0.9992806,0.00001880492,0.000004653248,0.0003133603],"study_design_scores_gemma":[0.000005455825,0.0001240384,0.002055598,0.000002364314,0.000007554492,0.00005416179,0.00001615236,0.001100291,0.996228,0.000009390866,0.0003927104,0.000004260744],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987603,0.0001242899,0.000783678,0.00001517988,0.000002897481,0.000005143567,0.00005816928,0.00001304889,0.0002373215],"genre_scores_gemma":[0.9988117,0.00008788954,0.0007720492,0.00001006625,8.869941e-7,0.000004928184,0.00006270332,0.000007284093,0.0002425313],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001362348,"threshold_uncertainty_score":0.002708793,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02777994314942209,"score_gpt":0.2715025826589156,"score_spread":0.2437226395094935,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}