{"id":"W4401443737","doi":"10.1007/s00253-024-13266-4","title":"CHO cell engineering via targeted integration of circular miR-21 decoy using CRISPR/RMCE hybrid system","year":2024,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Clinical Research Institute","funders":"Pasteur Institute of Iran","keywords":"Chinese hamster ovary cell; Expression cassette; CRISPR; Biology; Cell growth; Recombinant DNA; Cas9; Cell biology; Cell culture; Molecular biology; Gene; Genetics; Vector (molecular biology)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001372766,0.0002358304,0.0002668731,0.0001926637,0.00004793027,0.00001127338,0.0001583747,0.0005940928,0.000005284564],"category_scores_gemma":[0.00001025437,0.0002271728,0.00007223445,0.000155068,0.0001356502,0.000002922358,0.0001301562,0.0002349594,0.000006041223],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002243284,"about_ca_system_score_gemma":0.00003082055,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001340669,"about_ca_topic_score_gemma":0.000003224316,"domain_scores_codex":[0.9988816,0.00001536196,0.0002904497,0.0004823202,0.00002492018,0.000305308],"domain_scores_gemma":[0.9995854,0.00001497976,0.00005361429,0.0002785819,0.00002784025,0.0000395351],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001782846,0.00001486368,0.00001392205,0.0002429046,0.00007312284,0.000008577706,0.0000180817,0.0004341632,0.9962257,0.0006041371,0.00008959555,0.002257149],"study_design_scores_gemma":[0.0002321925,0.00008583864,0.00001896428,0.00004008006,0.00005208097,0.0003767392,0.00007187822,0.004097223,0.986868,0.00001742353,0.007911394,0.0002281952],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.651842,0.008090051,0.339335,0.0000411395,0.0002776219,0.0002358302,0.00003453296,0.0001033264,0.00004053702],"genre_scores_gemma":[0.9941758,0.0002270746,0.005261755,0.00002654037,0.00007314083,0.00002209121,0.0001624867,0.00003232741,0.00001878943],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3423338,"threshold_uncertainty_score":0.9263839,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003384700297647626,"score_gpt":0.2156738931263061,"score_spread":0.2122891928286584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}