{"id":"W4401443737","doi":"10.1007/s00253-024-13266-4","title":"CHO cell engineering via targeted integration of circular miR-21 decoy using CRISPR/RMCE hybrid system","year":2024,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Clinical Research Institute","funders":"Pasteur Institute of Iran","keywords":"Chinese hamster ovary cell; Expression cassette; CRISPR; Biology; Cell growth; Recombinant DNA; Cas9; Cell biology; Cell culture; Molecular biology; Gene; Genetics; Vector (molecular biology)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003538614,0.000420236,0.0005273444,0.0003912715,0.0002539859,0.0004859605,0.0004922283,0.0005403226,0.001275861],"category_scores_gemma":[0.0002234453,0.0002966752,0.0005100216,0.0003052014,0.0002581486,0.0002205855,0.0005249265,0.000737281,0.0009720714],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003352779,"about_ca_system_score_gemma":0.0003059159,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000788512,"about_ca_topic_score_gemma":0.001189461,"domain_scores_codex":[0.9995483,0.00005005245,0.00006188203,0.0001154572,0.0001629459,0.00006140538],"domain_scores_gemma":[0.9998652,0.00001923415,0.00004556234,0.00002571698,0.00002346997,0.00002089835],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003202481,0.00001636814,0.0001166398,0.00006818499,0.000009065029,0.0001145673,0.00003733436,0.00009880502,0.9972624,0.000274522,0.00009764319,0.001872503],"study_design_scores_gemma":[0.00001440468,0.000171964,0.001040517,0.000009329091,0.00003222208,0.0005080565,0.00002894134,0.001642736,0.9826915,0.00006264573,0.01377931,0.00001853034],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8382953,0.003489935,0.1396402,0.0005013904,0.0002946997,0.0005662436,0.002582001,0.001969346,0.01266086],"genre_scores_gemma":[0.8625536,0.003013624,0.1073949,0.0002480625,0.00003007512,0.0005896591,0.005037154,0.0003056175,0.02082737],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001275861,"threshold_uncertainty_score":0.004268229,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003384700297647626,"score_gpt":0.2156738931263061,"score_spread":0.2122891928286584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}