{"id":"W4401700566","doi":"10.2196/53175","title":"Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study","year":2024,"lang":"en","type":"article","venue":"JMIR Public Health and Surveillance","topic":"SARS-CoV-2 detection and testing","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"","keywords":"TaqMan; Digital polymerase chain reaction; Reverse transcriptase; Reverse transcription polymerase chain reaction; Polymerase chain reaction; Real-time polymerase chain reaction; Wastewater; RNA extraction; Biology; Virology; Molecular biology; Gene; RNA; Genetics; Gene expression; Environmental science","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002750811,0.000285363,0.001097744,0.0003952701,0.0001234813,0.00003633247,0.0000942689,0.0001121982,0.000006090885],"category_scores_gemma":[0.0005076046,0.0002345475,0.0001704577,0.0005736165,0.0001120918,0.0001170502,0.00002087326,0.0002974084,0.000001297544],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001026579,"about_ca_system_score_gemma":0.0004982043,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001486186,"about_ca_topic_score_gemma":0.0002065595,"domain_scores_codex":[0.9967574,0.0008411472,0.001063496,0.0005370838,0.0003062695,0.0004946391],"domain_scores_gemma":[0.9980562,0.0007791374,0.0002927689,0.0004202177,0.0002345189,0.000217154],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001072297,0.002112327,0.2427744,0.006222945,0.0002435531,0.00001146697,0.006882017,5.157166e-7,0.6526449,0.0001166779,0.003099642,0.08481922],"study_design_scores_gemma":[0.02128582,0.01511443,0.07741296,0.001072255,0.00005737564,0.0001082933,0.01705977,0.06242146,0.5497495,0.000121573,0.2538132,0.001783357],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9860092,0.002532136,0.005903888,0.002709247,0.0006636871,0.001828079,0.00008014848,0.0001932406,0.00008030888],"genre_scores_gemma":[0.9970919,0.00002863878,0.001228488,0.001119444,0.0001051071,0.0002942219,0.00005066914,0.00004654591,0.00003495055],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2507136,"threshold_uncertainty_score":0.9564568,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1487675206736524,"score_gpt":0.4394040737627444,"score_spread":0.290636553089092,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}