{"id":"W4401715584","doi":"10.1021/acschembio.4c00290","title":"<i>In Vivo</i> Injection of Reversible Optically Controlled Short Interfering RNA into Japanese Medaka Embryos (<i>Oryzias latipes</i>) to Regulate Gene Silencing","year":2024,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Tech University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Oryzias; Gene silencing; In vivo; Embryo; Small interfering RNA; Cell biology; Gene; Biology; RNA; RNA interference; Molecular biology; Chemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002908981,0.0002322428,0.0004431792,0.0001447011,0.0000240365,0.00001895713,0.0002993688,0.0003637512,0.00003914215],"category_scores_gemma":[0.0002116171,0.0002062292,0.0001610643,0.0002231261,0.000109404,0.0000129956,0.0002698445,0.0001926202,0.00002253592],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005014985,"about_ca_system_score_gemma":0.00006464861,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001303101,"about_ca_topic_score_gemma":0.0000431869,"domain_scores_codex":[0.9983822,0.00006517297,0.0005284572,0.0005900548,0.00009001483,0.0003440626],"domain_scores_gemma":[0.9993419,0.00007227431,0.00004717707,0.0003279306,0.0001011691,0.0001095425],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007384972,0.00003944402,0.0002489573,0.00004743887,0.0001097085,0.00001137213,0.0003076445,0.00009205356,0.9970484,0.00004216806,0.0005504265,0.0007638554],"study_design_scores_gemma":[0.0006042151,0.0005322776,0.0000292891,0.00008958011,0.00003166614,0.00003847848,0.0001033892,0.0002647945,0.9972538,0.0001650778,0.0006617705,0.0002256837],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9967626,0.0008560263,0.0005260532,0.0002873711,0.0004257859,0.0002634724,0.00001411488,0.00002473555,0.0008397925],"genre_scores_gemma":[0.9979421,0.0001619128,0.0007363888,0.0004606963,0.0002237333,0.00004984039,0.00004436588,0.00002410835,0.0003567968],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001179509,"threshold_uncertainty_score":0.8409783,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008529454369055755,"score_gpt":0.2590124407039074,"score_spread":0.2504829863348516,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}