{"id":"W4401715584","doi":"10.1021/acschembio.4c00290","title":"<i>In Vivo</i> Injection of Reversible Optically Controlled Short Interfering RNA into Japanese Medaka Embryos (<i>Oryzias latipes</i>) to Regulate Gene Silencing","year":2024,"lang":"en","type":"article","venue":"ACS Chemical Biology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Tech University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Oryzias; Gene silencing; In vivo; Embryo; Small interfering RNA; Cell biology; Gene; Biology; RNA; RNA interference; Molecular biology; Chemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001236715,0.0004313853,0.0001693571,0.0001357497,0.0001388692,0.000229492,0.0002745361,0.0002446554,0.001122396],"category_scores_gemma":[0.00006712334,0.0001841533,0.0002312472,0.00008113714,0.0002995298,0.000232105,0.0001470002,0.0004634486,0.0002921965],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003284693,"about_ca_system_score_gemma":0.0002198705,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001844642,"about_ca_topic_score_gemma":0.002609037,"domain_scores_codex":[0.9999101,0.000007828292,0.000007227908,0.0000372638,0.00001676561,0.00002065827],"domain_scores_gemma":[0.9999008,0.00001857906,0.00004296551,0.00001428576,0.00001094331,0.00001246165],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001129646,0.000003167818,0.00002296717,0.00001000798,9.527492e-7,0.000009562844,0.000005138278,0.00001328427,0.9996743,0.00006606321,0.00001330789,0.000169844],"study_design_scores_gemma":[0.000002336821,0.00002740101,0.0004344981,7.942328e-7,0.000003270905,0.00001865488,0.000003720537,0.0001245357,0.9989609,0.000007962915,0.0004146617,0.00000133939],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9808904,0.0006304302,0.01216268,0.0001245216,0.00005966019,0.000122417,0.0006988029,0.0004143581,0.004896721],"genre_scores_gemma":[0.9847642,0.0004308625,0.007162206,0.000103107,0.00001212866,0.0001073406,0.0004679636,0.0001211488,0.006831147],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001844642,"threshold_uncertainty_score":0.003754735,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008529454369055755,"score_gpt":0.2590124407039074,"score_spread":0.2504829863348516,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}