{"id":"W4401734418","doi":"10.1021/acs.bioconjchem.4c00345","title":"A Novel Confocal Scanning Protein–Protein Interaction Assay (PPI-CONA) Reveals Exceptional Selectivity and Specificity of CC0651, a Small Molecule Binding Enhancer of the Weak Interaction between the E2 Ubiquitin-Conjugating Enzyme CDC34A and Ubiquitin","year":2024,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute; Université de Montréal; Institute for Research in Immunology and Cancer","funders":"Medical Research Council; Canadian Institutes of Health Research; Hospital for Sick Children; Institute of Genetics; Wellcome Trust","keywords":"Chemistry; Small molecule; Ubiquitin; Enhancer; Protein–protein interaction; Target protein; Binding selectivity; Drug discovery; Enzyme; Biochemistry; Computational biology; Biophysics; Gene; Transcription factor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.000994625,0.000327625,0.0003303981,0.00004631462,0.0002055607,0.0001109352,0.0002452993,0.0002911993,0.0000587794],"category_scores_gemma":[0.0003259856,0.0002540316,0.0001619283,0.0001951062,0.0003968415,0.00002917147,0.0003455172,0.000505372,0.000001900622],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006574293,"about_ca_system_score_gemma":0.0001572283,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008482389,"about_ca_topic_score_gemma":0.00001533496,"domain_scores_codex":[0.9980568,0.0001707234,0.0005635231,0.000635246,0.000244653,0.0003290103],"domain_scores_gemma":[0.9988369,0.0001341035,0.0004280595,0.0003339482,0.0001738346,0.00009310499],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001095997,0.00004383987,0.0006214781,0.0006745623,0.0001283608,0.000001034617,0.0001752563,0.000004085603,0.9959937,0.000106047,0.00004707178,0.00209495],"study_design_scores_gemma":[0.0003988124,0.0001093726,0.0005520738,0.001006541,0.00005165063,0.0000653842,0.0004279017,0.0002456631,0.9958754,0.00009631483,0.0008997078,0.0002711578],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946339,0.0006521988,0.00199652,0.000302292,0.0001078506,0.0007705759,0.0001795344,0.000028711,0.001328447],"genre_scores_gemma":[0.9981054,0.00003101672,0.0003678183,0.00003091096,0.0004817642,0.0001530695,0.00007578715,0.00004077857,0.0007134562],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003471528,"threshold_uncertainty_score":0.9999912,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02446343374612983,"score_gpt":0.263799091205879,"score_spread":0.2393356574597492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}