{"id":"W4401734418","doi":"10.1021/acs.bioconjchem.4c00345","title":"A Novel Confocal Scanning Protein–Protein Interaction Assay (PPI-CONA) Reveals Exceptional Selectivity and Specificity of CC0651, a Small Molecule Binding Enhancer of the Weak Interaction between the E2 Ubiquitin-Conjugating Enzyme CDC34A and Ubiquitin","year":2024,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute; Université de Montréal; Institute for Research in Immunology and Cancer","funders":"Medical Research Council; Canadian Institutes of Health Research; Hospital for Sick Children; Institute of Genetics; Wellcome Trust","keywords":"Chemistry; Small molecule; Ubiquitin; Enhancer; Protein–protein interaction; Target protein; Binding selectivity; Drug discovery; Enzyme; Biochemistry; Computational biology; Biophysics; Gene; Transcription factor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004312739,0.0007565636,0.0004290205,0.0004230185,0.0003925687,0.0003627228,0.0006827843,0.0007430331,0.001653107],"category_scores_gemma":[0.0004186927,0.0003028446,0.0003242395,0.0003722557,0.0005662201,0.0003002629,0.0003263742,0.0009947082,0.000480172],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007252168,"about_ca_system_score_gemma":0.0006056426,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001255097,"about_ca_topic_score_gemma":0.002860765,"domain_scores_codex":[0.9991775,0.0001386662,0.00003875231,0.0002989206,0.0002464449,0.00009975416],"domain_scores_gemma":[0.999598,0.0001493025,0.00007017458,0.0000496892,0.00006274382,0.00007011183],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001869649,0.00001335326,0.00007964546,0.00001283914,0.000002972269,0.00001583827,0.00000406099,0.00003505709,0.9991115,0.00007107451,0.00002971378,0.0006052815],"study_design_scores_gemma":[0.000002866965,0.00006433669,0.00100953,0.000001053041,0.000003352659,0.0001424876,0.000003717414,0.0007170681,0.9974571,0.00001373805,0.0005807779,0.000003965629],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.890764,0.002090103,0.09686267,0.0003333562,0.00005396077,0.000267122,0.001071862,0.0007605955,0.007796324],"genre_scores_gemma":[0.8961693,0.001299835,0.09610797,0.0002149409,0.00001802821,0.0003415619,0.0018684,0.00007637335,0.003903547],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001653107,"threshold_uncertainty_score":0.005530179,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02446343374612983,"score_gpt":0.263799091205879,"score_spread":0.2393356574597492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}