{"id":"W4401868077","doi":"10.1021/jacs.4c02354","title":"RNA-Activated CRISPR/Cas12a Nanorobots Operating in Living Cells","year":2024,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":85,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"National Key Research and Development Program of China; Chinese Academy of Sciences; National Natural Science Foundation of China","keywords":"Trans-activating crRNA; CRISPR; RNA; Chemistry; Nanorobotics; Cleavage (geology); Ribonucleoprotein; Cas9; Guide RNA; Nucleic acid; Computational biology; Cell biology; Gene; Biology; Biochemistry; Nanotechnology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000288787,0.0003752459,0.000488612,0.0001444195,0.0002460949,0.0004213919,0.000490486,0.000746794,0.0006997875],"category_scores_gemma":[0.0002605563,0.0002928709,0.0002974521,0.0001291439,0.0004264533,0.000480895,0.0003210764,0.0005804443,0.000655832],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004875687,"about_ca_system_score_gemma":0.0002528281,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008137868,"about_ca_topic_score_gemma":0.001194688,"domain_scores_codex":[0.9996858,0.00004151565,0.00002439311,0.0001249596,0.00008364355,0.00003967413],"domain_scores_gemma":[0.9998487,0.00004212618,0.00003144741,0.00002230334,0.0000326278,0.00002265835],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002002977,0.000007493043,0.0000322255,0.00003961786,0.000002342239,0.00004268123,0.00002302286,0.0002912669,0.9982383,0.0003537268,0.00004473513,0.0009044919],"study_design_scores_gemma":[0.000006070935,0.00005221746,0.0001765763,0.000003430396,0.000003231472,0.00004847043,0.00001375721,0.002338353,0.9957058,0.0001115356,0.001532897,0.000007724851],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8586041,0.002503028,0.1283675,0.0002971689,0.0003004632,0.0002338258,0.0006269893,0.001285298,0.007781487],"genre_scores_gemma":[0.881137,0.001045367,0.1088329,0.000198395,0.00003027643,0.0003074609,0.000465214,0.0001063917,0.007877073],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008137868,"threshold_uncertainty_score":0.003537536,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006099311789054297,"score_gpt":0.2866974258080301,"score_spread":0.2805981140189758,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}