{"id":"W4402267440","doi":"10.1128/jb.00307-24","title":"Overexpression of diglucosyldiacylglycerol synthase leads to daptomycin resistance in <i>Bacillus subtilis</i>","year":2024,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Institute of Genetics; Ichiro Kanehara Foundation for the Promotion of Medical Sciences and Medical Care; Japan Society for the Promotion of Science; Ministry of Education, Culture, Sports, Science and Technology; Ryobi Teien Memory Foundation; Japan Agency for Medical Research and Development; Takeda Science Foundation","keywords":"Daptomycin; Lipopeptide; Bacillus subtilis; Microbiology; Biology; Bacteria; Phosphatidylglycerol; Surfactin; Cardiolipin; Mutant; Minimum inhibitory concentration; Phospholipid; Antibiotics; Staphylococcus aureus; Biochemistry; Gene; Membrane; Vancomycin; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001443712,0.00044729,0.000348884,0.0002123048,0.000121688,0.0004255676,0.0002154102,0.0002819574,0.0007614553],"category_scores_gemma":[0.0001916433,0.00021361,0.0002825683,0.0002027539,0.0001835035,0.0001159357,0.0003105951,0.0005849556,0.0005929495],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003785134,"about_ca_system_score_gemma":0.0001871892,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00158172,"about_ca_topic_score_gemma":0.0009987943,"domain_scores_codex":[0.9997546,0.00004703269,0.00004308268,0.00003970901,0.00007158543,0.00004405055],"domain_scores_gemma":[0.9997149,0.00006003264,0.00009677386,0.00002540498,0.00002694604,0.00007593956],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003930937,0.0000140136,0.00006928278,0.00001351179,0.000002339427,0.00003281776,0.000007447492,0.00003048287,0.9995369,0.00002322728,0.00001051603,0.0002202115],"study_design_scores_gemma":[0.00001274308,0.0001653065,0.002557153,0.00000929477,0.00001211374,0.0002235929,0.00004159609,0.001436834,0.9943522,0.00001892493,0.001162023,0.000008099284],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9947672,0.0004783067,0.002918583,0.0001093914,0.00003285703,0.00003258679,0.0005314623,0.0001624722,0.0009671284],"genre_scores_gemma":[0.9924814,0.0003266374,0.003425517,0.00004896612,0.000009768179,0.00003739515,0.001347336,0.0001047191,0.002218284],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00158172,"threshold_uncertainty_score":0.003145039,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007639197121056878,"score_gpt":0.2442237643561259,"score_spread":0.2365845672350691,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}