{"id":"W4402479373","doi":"10.1021/acs.analchem.4c03316","title":"Protein-Templated Click Ligation Reaction Triggered by Protein-Split Aptamer Interactions","year":2024,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Fundamental Research Funds for the Central Universities; State Key Laboratory of Urban Water Resource and Environment; National Natural Science Foundation of China","keywords":"Chemistry; Click chemistry; Aptamer; Ligation; Combinatorial chemistry; Chemical ligation; Biochemistry; Molecular biology; Amino acid","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006168084,0.0006761994,0.0004605225,0.0002795569,0.0001550802,0.0004009597,0.0005491415,0.0007547706,0.001133185],"category_scores_gemma":[0.0006873872,0.0003553436,0.000469281,0.0002761137,0.0004597917,0.0005676334,0.0005973106,0.0008872494,0.0007951963],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003191552,"about_ca_system_score_gemma":0.000265622,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001200908,"about_ca_topic_score_gemma":0.000195265,"domain_scores_codex":[0.9993334,0.0001134449,0.00005670618,0.0001815621,0.0002315984,0.0000832187],"domain_scores_gemma":[0.9996363,0.00009569874,0.0001256838,0.0000402693,0.00004369886,0.00005826712],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005197543,0.00003471982,0.00009156539,0.00006843741,0.000009668701,0.0001250267,0.00002757221,0.0002369948,0.9951984,0.0004684432,0.0001203045,0.003566975],"study_design_scores_gemma":[0.000006951325,0.0001190303,0.0001311946,0.000001200449,0.000003997357,0.0001901282,0.000003373539,0.001462189,0.9970815,0.00004002704,0.0009541384,0.00000637991],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7941299,0.00230934,0.1964919,0.0003774335,0.0001940542,0.0002695199,0.000308692,0.001731642,0.004187437],"genre_scores_gemma":[0.9424732,0.0008423808,0.05195188,0.0001766523,0.00004228423,0.00016174,0.0003986222,0.00006717454,0.003885985],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001133185,"threshold_uncertainty_score":0.003790855,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0105471510724393,"score_gpt":0.2917322297778201,"score_spread":0.2811850787053808,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}