{"id":"W4402519884","doi":"10.1039/d4cc03922j","title":"Serine 85 functions as a catalytic acid in the reprotonation process during EvAS-catalyzed astellifadiene biosynthesis","year":2024,"lang":"en","type":"article","venue":"Chemical Communications","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"National Key Research and Development Program of China; Higher Education Discipline Innovation Project; Science and Technology Commission of Shanghai Municipality; National Natural Science Foundation of China","keywords":"Catalysis; Biosynthesis; Serine; Chemistry; Biochemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001359606,0.0001982288,0.0001461317,0.00008463331,0.000131827,0.0002000308,0.0001397677,0.0002070025,0.001347066],"category_scores_gemma":[0.0001815713,0.00005847756,0.0001538475,0.0001382616,0.0001684013,0.0003079347,0.0001947212,0.000383685,0.0005009937],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002356298,"about_ca_system_score_gemma":0.0002298565,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005974157,"about_ca_topic_score_gemma":0.0006333988,"domain_scores_codex":[0.9999229,0.0000145143,0.000005676169,0.00001338743,0.00001840481,0.00002514128],"domain_scores_gemma":[0.9999328,0.00001063085,0.00001984974,0.000009186067,0.00001006643,0.00001747694],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003152658,0.00004052486,0.0009815232,0.00004705739,0.000006993149,0.0001989748,0.00004665295,0.0004818754,0.993812,0.0005139507,0.00006583433,0.003489378],"study_design_scores_gemma":[0.0000127845,0.0001478822,0.002653691,0.000004186089,0.000006135732,0.0001450385,0.00003915764,0.001186069,0.9937159,0.0001071431,0.001975719,0.000006280722],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9962398,0.0002906147,0.001927929,0.00007927881,0.00002004754,0.00001162795,0.0001388284,0.00003180871,0.001260068],"genre_scores_gemma":[0.9971386,0.0001510475,0.001145657,0.00002726831,0.000002289217,0.000005442221,0.000254518,0.000007483304,0.001267754],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001347066,"threshold_uncertainty_score":0.004506409,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01593238513694354,"score_gpt":0.2780935956905163,"score_spread":0.2621612105535727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}