{"id":"W4402575667","doi":"10.1002/btpr.3506","title":"Fed‐batch strategies for intensified rVSV vector production in high cell density cultures of suspension HEK293 cells","year":2024,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Polytechnique Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Vesicular stomatitis virus; HEK 293 cells; Titer; Fed-batch culture; Cell culture; Bioreactor; Biology; Viral vector; Recombinant DNA; Suspension culture; Yield (engineering); Suspension (topology); Chemistry; Virology; Virus; Biochemistry; Gene; Fermentation; Genetics; Materials science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004172615,0.0006669053,0.0006134587,0.0002184602,0.000181428,0.0006559817,0.0005785575,0.0005188791,0.0007117148],"category_scores_gemma":[0.0002373531,0.0001968978,0.0005237622,0.0002296135,0.0002062096,0.000374992,0.0004248474,0.0008916109,0.0006501238],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003648912,"about_ca_system_score_gemma":0.0002583789,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008549734,"about_ca_topic_score_gemma":0.0009610833,"domain_scores_codex":[0.9997365,0.00004253992,0.00003188421,0.00006318139,0.00009356198,0.00003233077],"domain_scores_gemma":[0.9998667,0.00002989983,0.00003589386,0.00002022665,0.00003011808,0.00001715419],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003723082,0.00004065426,0.0001205791,0.00002462971,0.00000434306,0.00003058128,0.00001043294,0.0001350677,0.9985421,0.00003651321,0.00004236892,0.000975566],"study_design_scores_gemma":[0.000005315071,0.0001706266,0.000704799,0.00000372757,0.00001040553,0.00006497437,0.00001050709,0.001760169,0.9966317,0.00001739643,0.0006132721,0.000006961262],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9541497,0.0005857854,0.04235514,0.0001092784,0.0000683716,0.0001885073,0.0008062039,0.0005029782,0.001234063],"genre_scores_gemma":[0.9243426,0.0008054946,0.06899751,0.00006751885,0.00002788432,0.0002537121,0.001951785,0.0001077383,0.003445784],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008549734,"threshold_uncertainty_score":0.00264746,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0148079956263346,"score_gpt":0.2850713091457386,"score_spread":0.270263313519404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}