{"id":"W4402575667","doi":"10.1002/btpr.3506","title":"Fed‐batch strategies for intensified rVSV vector production in high cell density cultures of suspension HEK293 cells","year":2024,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Polytechnique Montréal","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Vesicular stomatitis virus; HEK 293 cells; Titer; Fed-batch culture; Cell culture; Bioreactor; Biology; Viral vector; Recombinant DNA; Suspension culture; Yield (engineering); Suspension (topology); Chemistry; Virology; Virus; Biochemistry; Gene; Fermentation; Genetics; Materials science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0005141274,0.0003668614,0.0004250176,0.0003545843,0.000116177,0.00007994282,0.000510464,0.001143195,0.000002376634],"category_scores_gemma":[0.0003142538,0.0003299803,0.0001594691,0.000556601,0.0006737069,0.00001933892,0.0002825095,0.0004859975,0.00001703247],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006134591,"about_ca_system_score_gemma":0.0002837895,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004718762,"about_ca_topic_score_gemma":0.0003864497,"domain_scores_codex":[0.9974677,0.0001296343,0.0004285764,0.001032365,0.0002524227,0.0006893141],"domain_scores_gemma":[0.9985949,0.00008323782,0.0001509587,0.0007424907,0.0003508453,0.00007759261],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002430485,0.0002194319,0.0003332595,0.0003384587,0.0001006185,0.00002541901,0.000210124,0.00004276989,0.9875876,0.0004287864,0.007932324,0.002538124],"study_design_scores_gemma":[0.0006180426,0.002475912,0.000362852,0.00006981958,0.00003263183,0.00002130695,0.001415436,0.00009836982,0.9842634,0.0007064225,0.009841839,0.00009392702],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9895556,0.007224063,0.000179849,0.0008345632,0.0006719157,0.001259467,0.00004557643,0.0001768072,0.00005217112],"genre_scores_gemma":[0.9963398,0.0008844521,0.001210707,0.00005031527,0.0002370531,0.0001711665,0.0001568618,0.00007061745,0.0008790385],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006784201,"threshold_uncertainty_score":0.9999152,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0148079956263346,"score_gpt":0.2850713091457386,"score_spread":0.270263313519404,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}