{"id":"W4402642014","doi":"10.3390/mps7050074","title":"Co-Culture of Gut Bacteria and Metabolite Extraction Using Fast Vacuum Filtration and Centrifugation","year":2024,"lang":"en","type":"article","venue":"Methods and Protocols","topic":"Gut microbiota and health","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Trent University; University of Derby; Nottingham Trent University","keywords":"Metabolite; Extraction (chemistry); Bacteria; Filtration (mathematics); Centrifugation; Chromatography; Microbiology; Chemistry; Biology; Biochemistry; Mathematics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001137927,0.001653237,0.00105739,0.001416683,0.0009479116,0.00079667,0.0009095069,0.0007670266,0.002727521],"category_scores_gemma":[0.0008200641,0.0005484011,0.0007213613,0.0009567154,0.0003806453,0.0006373139,0.001257508,0.001247444,0.00448687],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002306501,"about_ca_system_score_gemma":0.0007786026,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005633545,"about_ca_topic_score_gemma":0.001367971,"domain_scores_codex":[0.998381,0.0002428775,0.0002216985,0.0003616254,0.0005541159,0.0002385891],"domain_scores_gemma":[0.9994568,0.000109393,0.00009793564,0.0001345936,0.0001482366,0.00005307781],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006439602,0.00004681896,0.0003023951,0.0002479448,0.00001890676,0.0001979958,0.00006704639,0.0001100448,0.9851377,0.0003522771,0.000650804,0.01280368],"study_design_scores_gemma":[0.00002458717,0.0004336,0.004459599,0.0001771744,0.00009657022,0.0009074273,0.00008364505,0.001242644,0.9210421,0.0004955155,0.07096402,0.00007298378],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2080444,0.01490494,0.7324519,0.0007842013,0.00130595,0.008525209,0.008206702,0.004262848,0.02151373],"genre_scores_gemma":[0.2109846,0.01087358,0.7243704,0.0005147372,0.0002774606,0.009836499,0.01887352,0.0008642162,0.02340496],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002727521,"threshold_uncertainty_score":0.009124398,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0369325847938258,"score_gpt":0.4601752619599601,"score_spread":0.4232426771661343,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}