{"id":"W4402784693","doi":"10.1002/advs.202402936","title":"A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations","year":2024,"lang":"en","type":"article","venue":"Advanced Science","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Vancouver Biotech (Canada)","funders":"","keywords":"Biology; Molecular biology; Cell biology; Protein biosynthesis; Innate immune system; Messenger RNA; Immune system; Gene; Biochemistry; Immunology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000147114,0.0001243329,0.00007837267,0.00005068066,0.0002132645,0.0001005637,0.0001827841,0.00004253044,0.000004384768],"category_scores_gemma":[0.00006182287,0.00009865821,0.00002047778,0.0002603866,0.000256672,0.00004009235,0.00008270361,0.00007415246,0.000004282451],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002062313,"about_ca_system_score_gemma":0.0001554228,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009165891,"about_ca_topic_score_gemma":0.00001151293,"domain_scores_codex":[0.9989167,0.00001224472,0.0001101017,0.0005385622,0.0001755349,0.000246872],"domain_scores_gemma":[0.9995593,0.00001309365,0.00003454766,0.0002057521,0.00007711291,0.000110183],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003887168,0.00002719929,0.00002256819,0.00001606434,0.000005203924,0.000002252423,0.0001015338,0.00006260524,0.997681,0.00003557307,0.00002653211,0.001980606],"study_design_scores_gemma":[0.000256556,0.0002269818,0.0001477925,0.0001241981,0.000004780746,0.00001880615,0.0001153776,0.0004159376,0.9979697,0.00008569443,0.0004762499,0.000157929],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8856586,0.0004658979,0.1131958,0.00004393093,0.00003284325,0.000231223,0.00002033752,0.00003586584,0.0003154791],"genre_scores_gemma":[0.9766667,0.00003977511,0.02286287,0.00008742444,0.00002334349,0.00007894908,0.00001713386,0.0000109047,0.0002128876],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09100813,"threshold_uncertainty_score":0.4023165,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006486462894175723,"score_gpt":0.2565527251740823,"score_spread":0.2500662622799066,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}