{"id":"W4403171009","doi":"10.1097/pai.0000000000001228","title":"A Modified Bleaching Method for Multiplex Immunofluorescence Staining of FFPE Tissue Sections","year":2024,"lang":"en","type":"article","venue":"Applied immunohistochemistry & molecular morphology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research; St. Michael's Hospital; Sunnybrook Health Science Centre","funders":"","keywords":"Staining; Chemistry; Multiplex; Hydrogen peroxide; Fluorophore; Stain; Fluorescence; Biomedical engineering; Pathology; Biochemistry; Medicine; Biology; Optics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001231486,0.00106578,0.0004122407,0.001121759,0.0004657355,0.0004230921,0.0007894016,0.0009673422,0.002845042],"category_scores_gemma":[0.0007354306,0.0008525378,0.0005363045,0.0005780713,0.0004164286,0.0004982048,0.00041549,0.001412102,0.001686145],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003125053,"about_ca_system_score_gemma":0.0004498415,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003911069,"about_ca_topic_score_gemma":0.001196641,"domain_scores_codex":[0.9990558,0.000144445,0.00009214551,0.0003284439,0.0002858448,0.00009326976],"domain_scores_gemma":[0.9993979,0.0001425647,0.0001149658,0.0001577386,0.000143366,0.00004353555],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002052936,0.00001347822,0.00007066526,0.00008790091,0.000005815951,0.00005304236,0.00002991716,0.00005026861,0.994905,0.0001695977,0.0001685831,0.004425338],"study_design_scores_gemma":[0.00001615637,0.0001838631,0.002560821,0.00002741396,0.00003998809,0.001454433,0.00001708426,0.002743033,0.9751033,0.0001738707,0.01763565,0.00004435033],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1378212,0.003215371,0.8504711,0.0002401158,0.0003550391,0.001105308,0.0007063543,0.002642241,0.003443273],"genre_scores_gemma":[0.09584211,0.002204272,0.8930727,0.00020958,0.00007698355,0.001903963,0.001086124,0.0002086795,0.005395546],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002845042,"threshold_uncertainty_score":0.00951761,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009075091597594629,"score_gpt":0.3236281632302453,"score_spread":0.3145530716326507,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}