{"id":"W4403235061","doi":"10.1111/febs.17295","title":"Biochemical plasticity of the <i>Escherichia coli</i><scp>CRISPR</scp> Cascade revealed by <i>in vitro</i> reconstitution of Cascade activities from purified Cas proteins","year":2024,"lang":"en","type":"article","venue":"FEBS Journal","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"U.S. National Library of Medicine; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; University of Toronto","keywords":"Trans-activating crRNA; CRISPR; Effector; Nuclease; Biology; DNA; RNA; Escherichia coli; Cell biology; Molecular biology; Cas9; Chemistry; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001841206,0.0001745461,0.0002397984,0.00005353395,0.00005647394,0.00003271326,0.0002212229,0.0002165454,0.000007175658],"category_scores_gemma":[0.0002095389,0.0001392078,0.0001671583,0.0001642162,0.0001436302,0.000009755151,0.00007545843,0.0004277522,9.589126e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004078971,"about_ca_system_score_gemma":0.0001891674,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001120835,"about_ca_topic_score_gemma":0.00003075414,"domain_scores_codex":[0.998842,0.0000780371,0.0004005927,0.00023148,0.000197788,0.0002500805],"domain_scores_gemma":[0.9995059,0.00006994082,0.0001259774,0.0001727972,0.00004268125,0.0000826368],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004936082,0.00004920581,0.0003733571,0.00006984617,0.00008051264,0.000008845391,0.0001872998,0.000255535,0.9935515,0.000008440769,0.005146533,0.0002195737],"study_design_scores_gemma":[0.0003909347,0.00006859758,0.001449725,0.0001672447,0.00004789177,0.00008173056,0.0001556617,0.0002836423,0.9901221,0.0000446766,0.007111631,0.00007611392],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9848567,0.005460195,0.008464387,0.00007564358,0.0004718224,0.0001966522,0.0002047965,0.000008295525,0.0002615023],"genre_scores_gemma":[0.9982798,0.0004054955,0.000750408,0.00004043246,0.0002726237,0.00001295147,0.00002343861,0.00002037326,0.0001944869],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01342308,"threshold_uncertainty_score":0.567673,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006581065891873058,"score_gpt":0.2504167453560889,"score_spread":0.2438356794642158,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}