{"id":"W4403396293","doi":"10.26685/urncst.649","title":"CRISPR-Mediated Transcriptional Activation of Squalene Synthase to Increase the Biosynthesis of Anticancer Metabolite Inotodiol in Inonotus Obliquus: A Research Protocol","year":2024,"lang":"en","type":"article","venue":"Undergraduate Research in Natural and Clinical Science and Technology (URNCST) Journal","topic":"Plant biochemistry and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"","keywords":"Squalene; Inonotus obliquus; CRISPR; Metabolite; Chemistry; Biosynthesis; ATP synthase; Biology; Biochemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004677015,0.0006677706,0.0006718218,0.0003869092,0.0003741498,0.000626658,0.0005870056,0.0007708612,0.002836268],"category_scores_gemma":[0.0003273832,0.0003675189,0.0006996968,0.0003437672,0.0003352798,0.0003785884,0.0006527387,0.0009483629,0.001396863],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004662446,"about_ca_system_score_gemma":0.0008054459,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009854242,"about_ca_topic_score_gemma":0.001635646,"domain_scores_codex":[0.9995028,0.00006046896,0.000076156,0.0001286205,0.0001490212,0.00008289819],"domain_scores_gemma":[0.9997788,0.00003552748,0.00005343596,0.00004266547,0.00004572974,0.00004382998],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000111786,0.00006214137,0.0001369319,0.0002626544,0.000008615992,0.0001986079,0.00007292008,0.0003217674,0.9937914,0.0005162659,0.000408798,0.004108026],"study_design_scores_gemma":[0.00006794916,0.0006450564,0.001000735,0.00004299537,0.00005878677,0.0004203695,0.00008525555,0.00193792,0.9641945,0.0003121902,0.03119089,0.00004340356],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.7269794,0.00871985,0.1999444,0.001885261,0.001151695,0.007671496,0.01324184,0.003815562,0.03659058],"genre_scores_gemma":[0.8180531,0.00648969,0.1251427,0.0005909065,0.00006234907,0.003533432,0.01369908,0.0004030678,0.03202583],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.002836268,"threshold_uncertainty_score":0.009488225,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05791679873783614,"score_gpt":0.4351306998236151,"score_spread":0.3772139010857789,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}