{"id":"W4403680022","doi":"10.1002/chem.202403435","title":"An Ultrahigh Affinity DNA Aptamer for Quinine and Its Intrinsic Fluorescence Based Label‐Free Detection","year":2024,"lang":"en","type":"article","venue":"Chemistry - A European Journal","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University; University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Isothermal titration calorimetry; Systematic evolution of ligands by exponential enrichment; Chemistry; Quinine; Fluorescence; Quenching (fluorescence); Combinatorial chemistry; Binding site; Biophysics; Biochemistry; Biology; Molecular biology; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000362612,0.0003937049,0.0003059889,0.0002841658,0.0001964728,0.0002596036,0.00049735,0.0006621194,0.000975015],"category_scores_gemma":[0.0003412371,0.0002384992,0.0002204493,0.0001564475,0.0003357729,0.0002485074,0.0003139569,0.0004995615,0.0004813078],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004750605,"about_ca_system_score_gemma":0.0001897688,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004645246,"about_ca_topic_score_gemma":0.0007202739,"domain_scores_codex":[0.9996215,0.00007215839,0.00002046841,0.0001177741,0.0001216822,0.00004635054],"domain_scores_gemma":[0.9997879,0.0000581547,0.00005101344,0.00002100431,0.00005473079,0.00002726472],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001217946,0.000007889508,0.00005260121,0.00001625404,0.00000203648,0.00001692058,0.00000755811,0.0000837775,0.9986499,0.00005054288,0.00001800298,0.001082398],"study_design_scores_gemma":[0.000003684131,0.00007138528,0.0002991749,0.000001546804,0.000003645031,0.00009147469,0.000004731949,0.001414382,0.9973283,0.00001892916,0.0007571973,0.000005559441],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8318552,0.001525564,0.1626507,0.0003786875,0.00007183715,0.0001384864,0.0002908356,0.000610046,0.002478579],"genre_scores_gemma":[0.9336833,0.0003972142,0.05883588,0.0001435927,0.00001384971,0.0001110694,0.0003347422,0.00003505876,0.006445299],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000975015,"threshold_uncertainty_score":0.003446817,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01312905205605866,"score_gpt":0.2630509712261989,"score_spread":0.2499219191701403,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}