{"id":"W4403683898","doi":"10.1002/ange.202414842","title":"Targeted Genome Mining Facilitates the Discovery of a Promiscuous, Hyperthermostable Amidase from <i>Thermovenabulum Gondwanense</i> with Notable Nylon‐Degrading Capacity","year":2024,"lang":"en","type":"article","venue":"Angewandte Chemie","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa; Queen's University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Amidase; Chemistry; Genome; Combinatorial chemistry; Computational biology; Biochemistry; Biology; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002615614,0.000387243,0.000334068,0.0003952243,0.0001670083,0.0006904878,0.0002769809,0.0004187458,0.0004569048],"category_scores_gemma":[0.0003413886,0.0001310715,0.0004083754,0.0006648619,0.000158834,0.0002184669,0.0003986261,0.0005125803,0.0003570224],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002648164,"about_ca_system_score_gemma":0.0003097169,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001106778,"about_ca_topic_score_gemma":0.001602627,"domain_scores_codex":[0.9998705,0.0000190678,0.00001345918,0.00003391366,0.00003925097,0.00002380576],"domain_scores_gemma":[0.9998609,0.00003428732,0.00004255909,0.0000198186,0.00002692192,0.00001548572],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004345742,0.00002408391,0.0008148422,0.00005012151,0.000008809388,0.000104025,0.00002558077,0.0001383167,0.9952695,0.00005350449,0.00004450933,0.003423331],"study_design_scores_gemma":[0.00001589193,0.0002209635,0.01646975,0.00002846318,0.0000631458,0.0008897849,0.0001678353,0.003340253,0.9718403,0.0001503024,0.006797549,0.00001576041],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.982178,0.0007874778,0.01308959,0.0002659362,0.00002330222,0.00008583423,0.002135135,0.0001493553,0.001285207],"genre_scores_gemma":[0.9698848,0.001067055,0.02125984,0.00009783793,0.00001294884,0.00007865683,0.005524155,0.00006512018,0.002009531],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001106778,"threshold_uncertainty_score":0.002200723,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01105941722092258,"score_gpt":0.1996183755470332,"score_spread":0.1885589583261107,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}