{"id":"W4404109930","doi":"10.21203/rs.3.rs-5325410/v1","title":"Streamlined Protoplast Transfection System for In-vivo Validation and Transgene-free Genome Editing in Banana","year":2024,"lang":"en","type":"preprint","venue":"Research Square","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"Optech (Canada)","funders":"","keywords":"Protoplast; Transgene; Genome editing; Transfection; In vivo; Genome; Biology; Computational biology; Cell biology; Gene; Biotechnology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000811925,0.0006829792,0.0007910915,0.0006698013,0.000663201,0.0008242453,0.0009204276,0.0009375138,0.005155283],"category_scores_gemma":[0.000453432,0.0006809137,0.0006429832,0.0005244904,0.0003715713,0.0005009324,0.0009893846,0.002352922,0.005437836],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005235648,"about_ca_system_score_gemma":0.0004308693,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001348338,"about_ca_topic_score_gemma":0.002224232,"domain_scores_codex":[0.999244,0.0001148487,0.00006642764,0.0002431793,0.0002342565,0.00009726266],"domain_scores_gemma":[0.9996117,0.0000948741,0.00004442226,0.0001362023,0.00006171497,0.00005112922],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004350983,0.00001548584,0.00005571545,0.00004391679,0.000004934244,0.00004458677,0.00004380539,0.0000743508,0.9967625,0.0003461797,0.0002562896,0.002308752],"study_design_scores_gemma":[0.00001903234,0.00003671561,0.0008371588,0.000009669478,0.00002465209,0.0002721319,0.0000222558,0.00196261,0.977208,0.0001856641,0.01940038,0.00002181269],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2647864,0.0009836046,0.6985736,0.0004848497,0.0004548283,0.001043934,0.01172044,0.01118456,0.01076767],"genre_scores_gemma":[0.4815406,0.001445318,0.4116881,0.0003973738,0.00007552288,0.001714928,0.03836806,0.00709619,0.05767381],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005155283,"threshold_uncertainty_score":0.01724619,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02331804422986986,"score_gpt":0.3695867992234548,"score_spread":0.3462687549935849,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}