{"id":"W4404196469","doi":"10.1002/smtd.202401572","title":"Dissociation Constant (<i>K</i><sub>d</sub>) Measurement for Small‐Molecule Binding Aptamers: Homogeneous Assay Methods and Critical Evaluations","year":2024,"lang":"en","type":"review","venue":"Small Methods","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Aptamer; Homogeneous; Dissociation constant; Small molecule; Dissociation (chemistry); Chemistry; Constant (computer programming); Binding constant; Molecule; Thermodynamics; Binding site; Physics; Physical chemistry; Molecular biology; Biology; Computer science; Biochemistry; Receptor; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001172204,0.001300845,0.001567928,0.002696238,0.0002956711,0.000992964,0.001141694,0.001595649,0.002143519],"category_scores_gemma":[0.001070656,0.000654502,0.0005779215,0.002515472,0.0007496539,0.001855323,0.000599525,0.002231896,0.002659689],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001095229,"about_ca_system_score_gemma":0.001005888,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009010659,"about_ca_topic_score_gemma":0.0009017496,"domain_scores_codex":[0.9994116,0.00008753358,0.00005083667,0.0001247156,0.0002858029,0.00003946825],"domain_scores_gemma":[0.999432,0.0002557484,0.00006563454,0.00001742722,0.0002033553,0.00002574564],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00008559824,0.0001375112,0.0002019001,0.02405025,0.00007991472,0.000221348,0.00009911707,0.001026194,0.02061281,0.01240902,0.02375118,0.9173251],"study_design_scores_gemma":[0.0000146146,0.0002310686,0.0006331686,0.001697649,0.00007721094,0.0009231934,0.00006651316,0.0003967221,0.01084787,0.00242329,0.982643,0.00004572094],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.000394274,0.995553,0.001358179,0.0002593845,0.0003540035,0.00001954364,0.00002991324,0.00001702235,0.00201469],"genre_scores_gemma":[0.002742041,0.9909966,0.002476356,0.0004326744,0.0002238475,0.00006987534,0.00006113462,0.000009089052,0.002988495],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.002696238,"threshold_uncertainty_score":0.007946551,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1301130184114086,"score_gpt":0.4801207326333298,"score_spread":0.3500077142219212,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}