{"id":"W4404398136","doi":"10.1002/cpz1.70051","title":"Injection of <i>Ortho</i>‐Functionalized Tetrafluorinated Azobenzene‐Containing siRNAs into Japanese Medaka Embryos for Photocontrolled Gene Silencing","year":2024,"lang":"en","type":"article","venue":"Current Protocols","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Tech University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Gene silencing; Oryzias; Embryo; Biology; Small interfering RNA; RNA interference; Cell biology; Gene; Green fluorescent protein; Transgene; Microinjection; Molecular biology; Genetics; Transfection; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001535394,0.0004635201,0.0002493839,0.0002264868,0.0002303813,0.0002766863,0.0003132286,0.0003297322,0.001914402],"category_scores_gemma":[0.00006929805,0.000282068,0.0003045509,0.0001170615,0.0002761819,0.0002516336,0.0002424877,0.0006169254,0.0008466512],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002878513,"about_ca_system_score_gemma":0.0002784395,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001071862,"about_ca_topic_score_gemma":0.002279883,"domain_scores_codex":[0.9998654,0.000008699892,0.00001383001,0.00005716157,0.00003430592,0.00002059314],"domain_scores_gemma":[0.9999241,0.00001276922,0.00002732806,0.00001314835,0.0000111574,0.00001150861],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000005939415,0.000001974519,0.00002243864,0.00001981357,0.000001063401,0.00001674488,0.000009145061,0.00001303511,0.9992654,0.00007325925,0.00001960258,0.0005515365],"study_design_scores_gemma":[0.00000340772,0.00005349143,0.0006623575,0.000004743597,0.000009236045,0.00009267018,0.000009079163,0.0002830277,0.9951433,0.00002074036,0.003713171,0.000004786746],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8739613,0.003464535,0.1005169,0.0003141358,0.0003221043,0.000512421,0.001639886,0.00148324,0.01778548],"genre_scores_gemma":[0.8836362,0.004114297,0.0796677,0.0002843381,0.0000523519,0.0005114605,0.001848143,0.0004703308,0.0294151],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001914402,"threshold_uncertainty_score":0.00640434,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03342280212338709,"score_gpt":0.3362578311428382,"score_spread":0.3028350290194511,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}