{"id":"W4404398136","doi":"10.1002/cpz1.70051","title":"Injection of <i>Ortho</i>‐Functionalized Tetrafluorinated Azobenzene‐Containing siRNAs into Japanese Medaka Embryos for Photocontrolled Gene Silencing","year":2024,"lang":"en","type":"article","venue":"Current Protocols","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ontario Tech University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Gene silencing; Oryzias; Embryo; Biology; Small interfering RNA; RNA interference; Cell biology; Gene; Green fluorescent protein; Transgene; Microinjection; Molecular biology; Genetics; Transfection; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001018084,0.0003561756,0.000580519,0.0001599071,0.0002080689,0.0001756929,0.000396403,0.0001678592,0.0005482351],"category_scores_gemma":[0.000354664,0.0003122033,0.0002865141,0.0003697083,0.0001247037,0.0004339762,0.00009751514,0.0002642278,0.00003907801],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002288361,"about_ca_system_score_gemma":0.0004482747,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000588893,"about_ca_topic_score_gemma":0.000005081389,"domain_scores_codex":[0.9973461,0.00008804668,0.0008804743,0.0007333443,0.0004668772,0.0004851526],"domain_scores_gemma":[0.9985691,0.0002777047,0.0002870962,0.0004032241,0.0003025794,0.0001602649],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009153212,0.0001552229,0.0001191286,0.001794226,0.0000366518,0.000004295464,0.0008498318,0.0001036818,0.9923235,0.0002107168,0.0003007636,0.003186634],"study_design_scores_gemma":[0.003043227,0.000372962,0.0000556405,0.00138769,0.00005789598,0.00002803241,0.0002540064,0.004628458,0.9838828,0.0007222922,0.005203105,0.0003638534],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8567968,0.0008207632,0.005580022,0.00003689987,0.001403884,0.1340738,0.0001290401,0.0005798463,0.0005788928],"genre_scores_gemma":[0.7158192,0.000005482662,0.0008236118,0.00001928469,0.0003920461,0.2826627,0.00005367403,0.00004946131,0.0001746131],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1485888,"threshold_uncertainty_score":0.999933,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03342280212338709,"score_gpt":0.3362578311428382,"score_spread":0.3028350290194511,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}