{"id":"W4404432045","doi":"10.1371/journal.ppat.1012118","title":"The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors","year":2024,"lang":"en","type":"article","venue":"PLoS Pathogens","topic":"Legionella and Acanthamoeba research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Innovation Cluster (Canada)","funders":"National Institute of Allergy and Infectious Diseases; Medizinischen Fakultät, Eberhard Karls Universität Tübingen; H2020 European Research Council; Agence Nationale de la Recherche; Fondation pour la Recherche Médicale; Deutsche Forschungsgemeinschaft","keywords":"Effector; Secretion; Cell biology; Periplasmic space; Legionella pneumophila; Transmembrane protein; Biology; Cytoplasm; Inner membrane; Membrane protein; Transmembrane domain; Secretory protein; Transport protein; Biochemistry; Membrane; Receptor; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000117473,0.0007549734,0.000399396,0.0002945134,0.0002638947,0.0006760203,0.0001699304,0.0004948768,0.0007167506],"category_scores_gemma":[0.000187642,0.0001935587,0.0007611404,0.0002911818,0.0002523888,0.0004829653,0.0004008747,0.0003729445,0.0006867733],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004052017,"about_ca_system_score_gemma":0.000333596,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009127689,"about_ca_topic_score_gemma":0.0006323632,"domain_scores_codex":[0.999818,0.00002796217,0.00002886663,0.00003953404,0.0000534348,0.00003225161],"domain_scores_gemma":[0.9998527,0.00001162289,0.00005786525,0.00001653701,0.00002506771,0.00003627298],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007327093,0.00001194379,0.001078268,0.00006091004,0.0000060798,0.0001442492,0.00001724991,0.00006507523,0.9975268,0.00008292752,0.00003226266,0.0009009552],"study_design_scores_gemma":[0.00002493488,0.0003487978,0.01641794,0.00002082003,0.00003473563,0.00114721,0.0001021559,0.001811284,0.9747473,0.0002266774,0.005087265,0.00003091567],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9871085,0.001780353,0.008468905,0.0001667808,0.0000414569,0.00006607876,0.0007263825,0.000339342,0.001302331],"genre_scores_gemma":[0.9861372,0.0007162251,0.008910025,0.00009082623,0.000009149508,0.00003741084,0.001572228,0.0000320722,0.002494871],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009127689,"threshold_uncertainty_score":0.002939999,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01118374302512349,"score_gpt":0.2581600333763551,"score_spread":0.2469762903512316,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}