{"id":"W4404660300","doi":"10.1111/gtc.13184","title":"Generation of Monosomy 21q Human <scp>iPS</scp> Cells by <scp>CRISPR</scp> /Cas9‐Mediated Interstitial Megabase Deletion","year":2024,"lang":"en","type":"article","venue":"Genes to Cells","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences; Institute of Genetics; Japan Agency for Medical Research and Development; Support for Pioneering Research Initiated by the Next Generation","keywords":"Biology; Monosomy; Genetics; Chromosome 21; Transcriptome; Cas9; CRISPR; Gene; Induced pluripotent stem cell; Ploidy; X-inactivation; Chromosome; Molecular biology; Cell biology; Embryonic stem cell; Gene expression; X chromosome; Karyotype","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001951871,0.0003301312,0.0002555789,0.0001512423,0.00008276953,0.00008706646,0.0002529814,0.000266495,0.00002135201],"category_scores_gemma":[0.00006026125,0.0003524739,0.0001746197,0.0002216385,0.0000531801,0.00001139836,0.0001495991,0.0001397455,0.0001049003],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003541478,"about_ca_system_score_gemma":0.00006114421,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005830452,"about_ca_topic_score_gemma":0.00004345419,"domain_scores_codex":[0.9981861,0.00006183954,0.000445884,0.0006295481,0.0002325929,0.000444001],"domain_scores_gemma":[0.9991315,0.00005612481,0.00007299218,0.0004079975,0.0001139458,0.0002174281],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000002595473,0.00004890325,0.00002974018,0.0001029597,0.00007098976,0.000009023378,0.0002613964,0.003927132,0.896171,0.00001127766,0.09811417,0.00125079],"study_design_scores_gemma":[0.0002352525,0.0003165235,0.00002488039,0.00002914368,0.00004981594,0.000005716505,0.0001473913,0.001960913,0.8076007,0.000005228124,0.1895441,0.00008031257],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9316188,0.0057534,0.0601129,0.00002926365,0.001183322,0.0003761786,0.0002253929,0.00006389927,0.0006368139],"genre_scores_gemma":[0.9926586,0.0006143545,0.001319112,0.0001618253,0.0009797598,0.00005378863,0.0009403627,0.0000808957,0.003191352],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09142993,"threshold_uncertainty_score":0.9998927,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01322786187091963,"score_gpt":0.2829114826768843,"score_spread":0.2696836208059646,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}