{"id":"W4404673350","doi":"10.1021/jacs.4c12585","title":"Circular Engineered Sortase for Interrogating Histone H3 in Chromatin","year":2024,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences; National Institute on Aging; NYU Grossman School of Medicine; Perelman School of Medicine, University of Pennsylvania; Nankai University; University of Leicester; University of Miami; York University; Thermo Fisher Scientific; University of Chicago; Wellcome Trust; Leonard M. Miller School of Medicine; University of Pennsylvania; Leukemia and Lymphoma Society; Charles A. King Trust; National Science Foundation; American Heart Association; National Cancer Institute; Brigham and Women's Hospital","keywords":"Sortase; Nucleosome; Histone; Chemistry; Chromatin; Epigenetics; Cell biology; Histone H3; Crosstalk; Proteomics; Computational biology; Biochemistry; Gene; Biology; Bacterial protein","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001262467,0.00009320726,0.0001617135,0.00001007492,0.00002049861,0.00001841406,0.0001899705,0.00006017721,0.000003942627],"category_scores_gemma":[0.0001247381,0.00006214318,0.0003650433,0.0001446662,0.0001091117,0.000005199764,0.00005456693,0.0001802676,2.792997e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000649189,"about_ca_system_score_gemma":0.00004425153,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003737947,"about_ca_topic_score_gemma":3.794805e-7,"domain_scores_codex":[0.9993547,0.00001570514,0.0002717957,0.0001233272,0.0001020782,0.0001324113],"domain_scores_gemma":[0.9996278,0.00002337038,0.0001610274,0.00009767096,0.00004701602,0.00004306772],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002546094,0.00001325833,0.00009318147,0.00004823918,0.00004840079,7.520108e-7,0.0001418241,0.00001109305,0.9963922,0.000004539721,0.001405041,0.001816009],"study_design_scores_gemma":[0.0002102083,0.00006737173,0.0004971244,0.00006707838,0.0000234097,0.00004289862,0.0001052387,0.001114075,0.9944189,0.0001076752,0.003251164,0.0000948118],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972373,0.0004787866,0.001138607,0.0009043541,0.0001491764,0.00006678888,0.00001381525,0.000004963827,0.000006205768],"genre_scores_gemma":[0.9967254,0.00004214187,0.00239519,0.0003784523,0.0003943356,0.000004090001,0.00002061578,0.00001306285,0.00002674228],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001973256,"threshold_uncertainty_score":0.2534125,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006529531536167644,"score_gpt":0.2583965382132783,"score_spread":0.2518670066771106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}