{"id":"W4404673350","doi":"10.1021/jacs.4c12585","title":"Circular Engineered Sortase for Interrogating Histone H3 in Chromatin","year":2024,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences; National Institute on Aging; NYU Grossman School of Medicine; Perelman School of Medicine, University of Pennsylvania; Nankai University; University of Leicester; University of Miami; York University; Thermo Fisher Scientific; University of Chicago; Wellcome Trust; Leonard M. Miller School of Medicine; University of Pennsylvania; Leukemia and Lymphoma Society; Charles A. King Trust; National Science Foundation; American Heart Association; National Cancer Institute; Brigham and Women's Hospital","keywords":"Sortase; Nucleosome; Histone; Chemistry; Chromatin; Epigenetics; Cell biology; Histone H3; Crosstalk; Proteomics; Computational biology; Biochemistry; Gene; Biology; Bacterial protein","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002030431,0.0002323129,0.0001127452,0.0001011656,0.00009768056,0.000258509,0.0001906571,0.0003423073,0.0006694911],"category_scores_gemma":[0.0001795958,0.000112816,0.0001267596,0.0001495156,0.0002410454,0.0002044155,0.0002066946,0.0004274112,0.0002342767],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002568198,"about_ca_system_score_gemma":0.000235755,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005052229,"about_ca_topic_score_gemma":0.001103008,"domain_scores_codex":[0.9998976,0.00001376232,0.00000833667,0.00003159604,0.00003334856,0.00001536135],"domain_scores_gemma":[0.9999021,0.0000242833,0.00002900986,0.00001408496,0.00001624597,0.00001431574],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002032556,0.00000619716,0.0001094319,0.00001198653,0.000001893121,0.00001242202,0.000004363167,0.00003585309,0.9986995,0.0001173541,0.00001897604,0.0009615886],"study_design_scores_gemma":[0.000002021301,0.00002783315,0.0002906092,6.361338e-7,0.000002050858,0.00004857118,0.000004807658,0.0004029019,0.9986446,0.00002049167,0.0005539648,0.000001419624],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9425279,0.0006726586,0.05471931,0.0001540447,0.00004760663,0.00004930875,0.0003429499,0.0001505377,0.001335607],"genre_scores_gemma":[0.965284,0.0006560513,0.03055649,0.0001101576,0.000006016327,0.00003573878,0.0005316084,0.00003385451,0.002786058],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006694911,"threshold_uncertainty_score":0.002239645,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006529531536167644,"score_gpt":0.2583965382132783,"score_spread":0.2518670066771106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}