{"id":"W4404701834","doi":"10.3390/pathogens13121034","title":"Development, Optimization, and Validation of a Quantitative PCR Assay for Borrelia burgdorferi Detection in Tick, Wildlife, and Human Samples","year":2024,"lang":"en","type":"article","venue":"Pathogens","topic":"Vector-borne infectious diseases","field":"Immunology and Microbiology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Atlantic Cancer Research Institute; Mount Allison University; Université de Moncton","funders":"Fondation canadienne de la maladie de Lyme","keywords":"Borrelia burgdorferi; Lyme disease; Biology; Borrelia; Tick; Virology; Real-time polymerase chain reaction; LYME; Primer (cosmetics); Polymerase chain reaction; Nested polymerase chain reaction; Microbiology; Gene; Genetics; Chemistry; Antibody","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002491339,0.0001006834,0.0001493231,0.000180499,0.0001062175,0.00001985355,0.00002726745,0.00009524026,0.00003041821],"category_scores_gemma":[0.0002138327,0.00009988674,0.00002436657,0.0001383933,0.00007878227,0.00008756875,0.00002336918,0.00005952634,0.000003848268],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003150539,"about_ca_system_score_gemma":0.00004818842,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000063351,"about_ca_topic_score_gemma":0.0001946802,"domain_scores_codex":[0.9993129,0.00009275229,0.0002269681,0.0002235241,0.0000184341,0.0001254683],"domain_scores_gemma":[0.9995703,0.0002245047,0.00005801464,0.00006462665,0.00006907181,0.00001349553],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009719696,0.0001336248,0.04221641,0.0003099511,0.0002506535,0.000002907406,0.00293944,0.000660255,0.9421211,0.004626128,0.0001956354,0.006446737],"study_design_scores_gemma":[0.0038381,0.001165024,0.2939002,0.0006093473,0.000545394,0.0001391543,0.002077917,0.003051458,0.6671119,0.002190203,0.02428509,0.001086244],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9473879,0.001754593,0.05012108,0.00002569736,0.0001864488,0.0003150417,0.0001057452,0.00005890959,0.0000446311],"genre_scores_gemma":[0.9964701,0.00006514935,0.003119757,0.00001684,0.000009708438,0.00007275543,0.0001863958,0.00001503465,0.00004427705],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2750092,"threshold_uncertainty_score":0.4073263,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02567401731172936,"score_gpt":0.2788169891310849,"score_spread":0.2531429718193555,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}