{"id":"W4404701834","doi":"10.3390/pathogens13121034","title":"Development, Optimization, and Validation of a Quantitative PCR Assay for Borrelia burgdorferi Detection in Tick, Wildlife, and Human Samples","year":2024,"lang":"en","type":"article","venue":"Pathogens","topic":"Vector-borne infectious diseases","field":"Immunology and Microbiology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Atlantic Cancer Research Institute; Mount Allison University; Université de Moncton","funders":"Fondation canadienne de la maladie de Lyme","keywords":"Borrelia burgdorferi; Lyme disease; Biology; Borrelia; Tick; Virology; Real-time polymerase chain reaction; LYME; Primer (cosmetics); Polymerase chain reaction; Nested polymerase chain reaction; Microbiology; Gene; Genetics; Chemistry; Antibody","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.009675172,0.001655458,0.0009307661,0.001680825,0.0005586324,0.001028919,0.0009489337,0.001267356,0.000755311],"category_scores_gemma":[0.00716407,0.0009416825,0.0009381764,0.0007331333,0.001049997,0.0005098692,0.0006653528,0.001175572,0.0007301171],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004786446,"about_ca_system_score_gemma":0.001059186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007149963,"about_ca_topic_score_gemma":0.001306328,"domain_scores_codex":[0.9879605,0.004284254,0.0009360383,0.002114412,0.004123088,0.0005818317],"domain_scores_gemma":[0.9948105,0.002033686,0.0004930474,0.0005524582,0.001997884,0.0001123515],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001496645,0.0002180873,0.003394862,0.0003056699,0.00004587658,0.00005419787,0.000229152,0.000894575,0.9768364,0.0003261351,0.0002601054,0.0172853],"study_design_scores_gemma":[0.00005820027,0.00172968,0.008921173,0.0001335206,0.0001658109,0.0005327056,0.0001606984,0.01359233,0.9639336,0.000516766,0.01016998,0.00008558786],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2583872,0.003679603,0.7292086,0.0003373683,0.0003509968,0.002415634,0.00180751,0.001141252,0.002671857],"genre_scores_gemma":[0.286981,0.001672459,0.6994209,0.0004524868,0.000152257,0.004339006,0.003376791,0.0002111967,0.003394],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.009675172,"threshold_uncertainty_score":0.05116785,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02567401731172936,"score_gpt":0.2788169891310849,"score_spread":0.2531429718193555,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}