{"id":"W4404772464","doi":"10.21769/bioprotoc.5162","title":"Cloning a Chloroplast Genome in &lt;em&gt;Saccharomyces cerevisiae&lt;/em&gt; and &lt;em&gt;Escherichia coli&lt;/em&gt;","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Saccharomyces cerevisiae; Cloning (programming); Genome; Chloroplast; Biology; Genetics; Yeast; Gene; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00109995,0.001217816,0.001408405,0.001141712,0.0007339396,0.000906352,0.001899717,0.0008071293,0.01115311],"category_scores_gemma":[0.0007723239,0.0008857989,0.001166266,0.002206153,0.0006771504,0.0006595873,0.0009152116,0.003282705,0.009464147],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008343704,"about_ca_system_score_gemma":0.001480277,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003757073,"about_ca_topic_score_gemma":0.01301574,"domain_scores_codex":[0.9993687,0.0001284581,0.00007450029,0.0001973539,0.0001395966,0.00009137198],"domain_scores_gemma":[0.9993802,0.0001637723,0.0000846868,0.0001396273,0.0001221979,0.0001094193],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002405471,0.0001360188,0.0002750513,0.000327097,0.00004166022,0.000129688,0.0001163198,0.0001257514,0.9858554,0.001384901,0.002477576,0.008890018],"study_design_scores_gemma":[0.000346824,0.0005274774,0.00839873,0.0001294293,0.0003034145,0.0005628709,0.0001885579,0.002331502,0.8152364,0.001261031,0.1706191,0.00009448008],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2457738,0.002918205,0.5922832,0.002318822,0.001684297,0.006818912,0.09705833,0.007706366,0.04343812],"genre_scores_gemma":[0.2192092,0.002915928,0.3071729,0.0009205542,0.0001497109,0.002116135,0.3383063,0.00383842,0.1253708],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01115311,"threshold_uncertainty_score":0.03731084,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009758783845602905,"score_gpt":0.2465603803696693,"score_spread":0.2368015965240663,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}