{"id":"W4404772464","doi":"10.21769/bioprotoc.5162","title":"Cloning a Chloroplast Genome in &lt;em&gt;Saccharomyces cerevisiae&lt;/em&gt; and &lt;em&gt;Escherichia coli&lt;/em&gt;","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Saccharomyces cerevisiae; Cloning (programming); Genome; Chloroplast; Biology; Genetics; Yeast; Gene; Computer science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0009265954,0.0008915357,0.0007838215,0.0003598898,0.0002486026,0.0004909081,0.0005591625,0.000730923,0.0002089772],"category_scores_gemma":[0.0002071352,0.0008267841,0.0002927725,0.000856214,0.0001569408,0.00005596035,0.0004363037,0.0005329478,0.0001402547],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001189276,"about_ca_system_score_gemma":0.0002389586,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003293635,"about_ca_topic_score_gemma":0.0002507261,"domain_scores_codex":[0.995608,0.0001796315,0.0009900866,0.001711144,0.0004568692,0.001054281],"domain_scores_gemma":[0.9982305,0.00002267188,0.0002647006,0.0009504488,0.0001942787,0.0003373736],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0003185667,0.0001744501,0.0001388974,0.0009828,0.0001593157,0.00004344899,0.0004016217,0.00009666043,0.9868132,0.0001860774,0.002874097,0.007810867],"study_design_scores_gemma":[0.00179325,0.0005800256,0.00331723,0.0003921816,0.00007871396,0.0001155175,0.0001077485,0.0003181764,0.2551149,0.00002705603,0.7371017,0.001053476],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8843064,0.002572954,0.0004818336,0.0002855175,0.0009866809,0.1103099,0.0001841541,0.0003115679,0.0005610724],"genre_scores_gemma":[0.7288636,0.0004402636,0.003855369,0.0003488187,0.005076052,0.2563373,0.0003515304,0.0003565498,0.004370459],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.7342276,"threshold_uncertainty_score":0.9994183,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009758783845602905,"score_gpt":0.2465603803696693,"score_spread":0.2368015965240663,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}