{"id":"W4404966814","doi":"10.1016/j.nbt.2024.12.001","title":"A rapid CAT transformation protocol and nuclear transgene expression tools for metabolic engineering in Cyanidioschyzon merolae 10D","year":2024,"lang":"en","type":"article","venue":"New Biotechnology","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Northern British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; King Abdullah University of Science and Technology; University of Northern British Columbia","keywords":"Transgene; Transformation (genetics); Protocol (science); Computational biology; Biology; Genetics; Gene; Medicine; Pathology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007517748,0.0008455213,0.0006601888,0.0008793331,0.0006602464,0.00046842,0.001035761,0.0004658292,0.003223189],"category_scores_gemma":[0.0004647851,0.0007715769,0.000633436,0.0007604079,0.0003326065,0.0003304346,0.0008885577,0.001432371,0.003758574],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006200886,"about_ca_system_score_gemma":0.0008442457,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001677718,"about_ca_topic_score_gemma":0.004059813,"domain_scores_codex":[0.999067,0.0001193366,0.0001674632,0.0002049665,0.0003419268,0.00009930576],"domain_scores_gemma":[0.9996433,0.00006809391,0.00005198653,0.0001028539,0.00009029137,0.00004345215],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004413169,0.00003837857,0.0001137942,0.0000991312,0.000005510954,0.00006974672,0.0000440644,0.00009208752,0.994601,0.0002966757,0.0004400456,0.004155522],"study_design_scores_gemma":[0.00003084273,0.000139759,0.001678725,0.00003438163,0.00002696515,0.0004097003,0.00003459509,0.001450118,0.9434302,0.0001450774,0.05258169,0.00003783444],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2187022,0.002052584,0.7407963,0.000589308,0.0004115436,0.005808543,0.01616989,0.005405091,0.01006448],"genre_scores_gemma":[0.2178068,0.00346249,0.6738638,0.0002999903,0.00004787897,0.008931497,0.05823863,0.002451494,0.03489738],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003223189,"threshold_uncertainty_score":0.0107826,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01239975818332866,"score_gpt":0.2477985262766768,"score_spread":0.2353987680933481,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}