{"id":"W4405808764","doi":"10.21769/bioprotoc.5178","title":"Using the <em>Sleeping Beauty</em> Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Phagocytosis and Immune Regulation","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"Canadian Institutes of Health Research","keywords":"Phagocytosis; Doxycycline; Sleeping Beauty transposon system; Transposable element; Gene; Macrophage; Gene expression; Biology; Molecular biology; Beauty; Cell biology; Microbiology; Genetics; Art; In vitro; Antibiotics; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000819378,0.001098981,0.0006840527,0.001053659,0.0007491283,0.0008279663,0.001190375,0.001121164,0.006742636],"category_scores_gemma":[0.0004036725,0.0005373345,0.0007680638,0.0007652245,0.0008517624,0.0005224444,0.0007732729,0.002831886,0.00786468],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007116348,"about_ca_system_score_gemma":0.001060212,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001619531,"about_ca_topic_score_gemma":0.003529635,"domain_scores_codex":[0.9990562,0.0001346478,0.000111909,0.0002201684,0.0003420253,0.000135005],"domain_scores_gemma":[0.9995632,0.00008549209,0.00009919953,0.0001205626,0.0000622126,0.00006935665],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005259892,0.00003709468,0.00005940967,0.0001256232,0.000008136381,0.00005877545,0.00003432281,0.00004977008,0.9957527,0.0005631171,0.0008310804,0.00242733],"study_design_scores_gemma":[0.00002002864,0.0001043341,0.0007158305,0.00002069041,0.00001446106,0.00023733,0.00001815395,0.0004441133,0.9766094,0.0001423052,0.02165638,0.00001701932],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.2763538,0.006521184,0.6384932,0.00244142,0.001776882,0.004425907,0.02549767,0.01134391,0.03314592],"genre_scores_gemma":[0.4894274,0.006307903,0.3532425,0.001492315,0.0001576432,0.005432615,0.04019446,0.003359828,0.1003854],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.006742636,"threshold_uncertainty_score":0.0225563,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04235645654776734,"score_gpt":0.3238904011299401,"score_spread":0.2815339445821727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}