{"id":"W4405808764","doi":"10.21769/bioprotoc.5178","title":"Using the &lt;em&gt;Sleeping Beauty&lt;/em&gt; Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis","year":2024,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Phagocytosis and Immune Regulation","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"Canadian Institutes of Health Research","keywords":"Phagocytosis; Doxycycline; Sleeping Beauty transposon system; Transposable element; Gene; Macrophage; Gene expression; Biology; Molecular biology; Beauty; Cell biology; Microbiology; Genetics; Art; In vitro; Antibiotics; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001769565,0.000527218,0.0006873449,0.0004103193,0.0006021587,0.0001815995,0.0006017265,0.0004595053,0.0001315798],"category_scores_gemma":[0.00003705575,0.0003858525,0.0003189985,0.0007008238,0.00006778937,0.0002891824,0.000171892,0.0003802195,0.0001360947],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003707453,"about_ca_system_score_gemma":0.0001316647,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006079755,"about_ca_topic_score_gemma":0.00005424243,"domain_scores_codex":[0.9961237,0.000745118,0.001108579,0.001000141,0.0001726044,0.0008498268],"domain_scores_gemma":[0.9985518,0.000215055,0.000232489,0.0008248377,0.0001071629,0.00006865909],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007745411,0.0004597032,0.00014889,0.0004652742,0.0001654957,0.00001872441,0.002591882,0.0003871956,0.984595,0.00003990821,0.0003309537,0.01002243],"study_design_scores_gemma":[0.004703862,0.0006220145,0.0009352282,0.0008162744,0.0001176344,0.00002813175,0.000981668,0.0009430162,0.9675974,0.00001538945,0.02278526,0.0004540849],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5622627,0.0005239855,0.007777036,0.00007427332,0.001131274,0.4278152,0.000100005,0.0002493999,0.00006614348],"genre_scores_gemma":[0.6210504,0.000001660291,0.0006965109,0.00003709426,0.0001609647,0.3777647,0.00002955266,0.00006851924,0.000190573],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05878775,"threshold_uncertainty_score":0.9998593,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04235645654776734,"score_gpt":0.3238904011299401,"score_spread":0.2815339445821727,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}