{"id":"W4406063250","doi":"10.1016/j.bios.2025.117137","title":"Genetically engineered integrated aflatoxin B1 and deoxynivalenol bispecific nanobody as surrogate antigens for constructed time-resolved immunoassay dual detection methods","year":2025,"lang":"en","type":"article","venue":"Biosensors and Bioelectronics","topic":"Mycotoxins in Agriculture and Food","field":"Agricultural and Biological Sciences","cited_by":15,"is_retracted":false,"has_abstract":false,"ca_institutions":"Ministry of Agriculture","funders":"National Natural Science Foundation of China; Department of Science and Technology of Shandong Province","keywords":"Immunoassay; Genetically engineered; Antigen; Aflatoxin; Genetically modified organism; Computational biology; Biology; Chemistry; Chromatography; Immunology; Biotechnology; Antibody; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003669164,0.0003123213,0.0003620929,0.00005342309,0.0003567576,0.0001434864,0.0001322707,0.0003412318,0.00005889674],"category_scores_gemma":[0.0001314294,0.0001340967,0.00011651,0.0006032793,0.0001660301,0.00005303675,0.00006616051,0.0002217097,0.00000523655],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004277271,"about_ca_system_score_gemma":0.00003235008,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004134139,"about_ca_topic_score_gemma":0.0001424716,"domain_scores_codex":[0.9983242,0.0001611997,0.0003497279,0.0005253982,0.0001177458,0.0005217517],"domain_scores_gemma":[0.9991783,0.000354502,0.00009231047,0.00008797638,0.0001756195,0.0001112675],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001313455,0.00004583421,0.0000576039,0.00001227348,0.00008756154,0.000001496477,0.00001922803,5.214125e-7,0.8786544,0.001037368,0.0001501002,0.1198023],"study_design_scores_gemma":[0.0007280942,0.001564388,0.004353757,0.00004472544,0.0001098622,0.00007005028,0.0002355903,0.001562372,0.8948435,0.001025077,0.09495921,0.0005033872],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946076,0.002993979,0.0005506642,0.0005393081,0.0001709933,0.0006411751,0.0001045518,0.0001361158,0.0002555875],"genre_scores_gemma":[0.9932387,0.001613564,0.003937231,0.0001229842,0.0001070196,0.00002879541,0.0001234399,0.000003804432,0.0008243895],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1192989,"threshold_uncertainty_score":0.5468302,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00680874967948287,"score_gpt":0.2410684432628832,"score_spread":0.2342596935834003,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}