{"id":"W4406150917","doi":"10.1021/acs.jchemed.4c01023","title":"Exploring Molecular Binding: A Fluorescence Anisotropy Lab for Undergraduates","year":2025,"lang":"en","type":"article","venue":"Journal of Chemical Education","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Fonds de Recherche du Québec - Santé; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Canada Foundation for Innovation; Génome Québec","keywords":"Aptamer; Fluorescence anisotropy; Nucleic acid; Fluorescence; Macromolecule; Chemistry; Biophysics; Biophysical chemistry; Nanotechnology; Biochemistry; Biology; Materials science; Physics; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002672766,0.001247395,0.0005574132,0.0007541271,0.00229895,0.002321023,0.002740445,0.001661151,0.02402791],"category_scores_gemma":[0.002504044,0.0005476646,0.0009166814,0.0004368667,0.00129475,0.002451634,0.005677451,0.003294899,0.01294932],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002160562,"about_ca_system_score_gemma":0.004193169,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007962939,"about_ca_topic_score_gemma":0.001910851,"domain_scores_codex":[0.9985671,0.0002810337,0.00004660297,0.0002715559,0.0005566999,0.0002770337],"domain_scores_gemma":[0.9949051,0.0002065846,0.0001564347,0.0005038806,0.001037719,0.003190239],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0006788987,0.007013721,0.01055516,0.000553647,0.00003096073,0.000621913,0.002167008,0.001932383,0.1436682,0.04946368,0.1661934,0.617121],"study_design_scores_gemma":[0.0001788201,0.004922734,0.007049649,0.0003577985,0.00005881764,0.003134118,0.002096118,0.01050656,0.1108694,0.03885607,0.821818,0.0001519275],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2156786,0.004372365,0.5364313,0.05733811,0.003613894,0.002149593,0.001980711,0.01546466,0.1629709],"genre_scores_gemma":[0.1824689,0.001963925,0.6876413,0.006575203,0.0008611027,0.0009908858,0.001075416,0.0008704408,0.1175528],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02402791,"threshold_uncertainty_score":0.08038139,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0255067170145922,"score_gpt":0.3186725814154096,"score_spread":0.2931658644008173,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}