{"id":"W4406595478","doi":"10.21769/bioprotoc.5210","title":"Real-time IncuCyte<sup>®</sup> Assay for the Dynamic Assessment of Live and Dead Cells in 2D Cultures","year":2025,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; Genome British Columbia; Simon Fraser University","funders":"Canadian Institutes of Health Research","keywords":"Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.008521244,0.01037051,0.01056695,0.007557051,0.004333069,0.006257982,0.009096141,0.003407783,0.1417229],"category_scores_gemma":[0.00318336,0.00609997,0.003568194,0.00820332,0.004984793,0.003727787,0.003397179,0.01892108,0.08019842],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.003285793,"about_ca_system_score_gemma":0.005382157,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001922748,"about_ca_topic_score_gemma":0.007822422,"domain_scores_codex":[0.9871475,0.002590488,0.001418096,0.004859424,0.002991043,0.0009934158],"domain_scores_gemma":[0.994844,0.001961573,0.0003652617,0.001386588,0.0008890396,0.0005536187],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001762134,0.0008254422,0.0005520309,0.005163828,0.0002667077,0.0002486224,0.001133262,0.001111053,0.8728625,0.01241558,0.06102593,0.04263289],"study_design_scores_gemma":[0.001170359,0.0009026934,0.002798858,0.0008801527,0.000341152,0.0008106505,0.0001679378,0.01495168,0.7326592,0.004541422,0.2403823,0.0003935802],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01500216,0.003723598,0.8682803,0.001395646,0.002378637,0.004496183,0.04395636,0.03091896,0.02984815],"genre_scores_gemma":[0.01624432,0.00331639,0.8057103,0.001093531,0.0005222414,0.01856525,0.07266974,0.01172924,0.07014897],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.1417229,"threshold_uncertainty_score":0.4741104,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01183527742797631,"score_gpt":0.3119995065967157,"score_spread":0.3001642291687394,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}