{"id":"W4406681799","doi":"10.1101/2025.01.19.633792","title":"Optimizing Transfection Efficiency in Primary Human Splenic Fibroblast Cells","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Kwantlen Polytechnic University","funders":"","keywords":"Transfection; Fibroblast; Primary (astronomy); Cell biology; Chemistry; Molecular biology; Biology; Cell culture; Genetics; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00174287,0.0004739693,0.0005515238,0.0003520294,0.0003488355,0.0005001304,0.0003491494,0.0004583695,0.0009923971],"category_scores_gemma":[0.000967472,0.0001655222,0.0003306082,0.0004784031,0.0003294764,0.0002219357,0.0002819774,0.0005089444,0.000601889],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005084727,"about_ca_system_score_gemma":0.0004414532,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002240065,"about_ca_topic_score_gemma":0.002706156,"domain_scores_codex":[0.9986726,0.0003532394,0.0001678565,0.0002746448,0.0003649955,0.000166713],"domain_scores_gemma":[0.9993548,0.0002722217,0.00007522891,0.0001069149,0.0001624343,0.00002848649],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006075942,0.00002202979,0.0001451261,0.00003668648,0.000003886877,0.00003241148,0.00004095969,0.0001281507,0.9977559,0.00007427466,0.00005487576,0.001644804],"study_design_scores_gemma":[0.000001838137,0.00006842359,0.0003809723,0.000002134369,0.000004633736,0.00002572951,0.000009012987,0.0002424016,0.9984033,0.00001446641,0.0008438246,0.000003482294],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.895701,0.003308135,0.09143664,0.0001854621,0.0001078735,0.0007343033,0.002019062,0.0007072031,0.00580034],"genre_scores_gemma":[0.8858712,0.003121725,0.1001543,0.0001299241,0.00002284641,0.000537237,0.001961445,0.0003409757,0.007860372],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002240065,"threshold_uncertainty_score":0.009217262,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008417146727887702,"score_gpt":0.2167120026760544,"score_spread":0.2082948559481667,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}