{"id":"W4406707721","doi":"10.1039/d4sc06819j","title":"Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study","year":2025,"lang":"en","type":"article","venue":"Chemical Science","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Azrieli Foundation; Israel Science Foundation; H2020 European Research Council; Weizmann Institute of Science; Deutsche Forschungsgemeinschaft; European Commission","keywords":"Förster resonance energy transfer; Single-molecule FRET; Substrate (aquarium); Chemistry; Biophysics; Enzyme; Dynamics (music); Molecule; Conformational change; Molecular dynamics; Stereochemistry; Computational chemistry; Biochemistry; Biology; Fluorescence; Physics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002329849,0.0001236785,0.000126312,0.00007189189,0.0001236526,0.00008646242,0.0003222115,0.00007429406,0.000002369504],"category_scores_gemma":[0.0001590191,0.0001156063,0.00002211673,0.0003030614,0.0006168536,0.00002660686,0.0003286103,0.0001049656,0.000001145612],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00008092169,"about_ca_system_score_gemma":0.00008334418,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004192128,"about_ca_topic_score_gemma":0.000004446007,"domain_scores_codex":[0.9990807,0.00001420414,0.0001627458,0.0003521504,0.0001469992,0.0002431846],"domain_scores_gemma":[0.9995552,0.0000250425,0.0000643286,0.0002162697,0.00006866449,0.00007051183],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001185044,0.00004442993,0.008063924,0.00001133761,0.000007536319,5.169518e-7,0.00004423098,6.425721e-7,0.989886,0.0001264458,0.00002706088,0.00177605],"study_design_scores_gemma":[0.000154089,0.00009155161,0.002425253,0.00003828393,0.000008707411,0.000003014012,0.0001136049,0.0006297837,0.9961026,0.0002931126,0.00001738364,0.0001225969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9801284,0.00002560366,0.01903877,0.00007386081,0.00002759968,0.0002113997,0.00001263243,0.00002870045,0.000453044],"genre_scores_gemma":[0.995266,0.000003190535,0.004553027,0.00004209463,0.00001511703,0.00001412134,0.00002343042,0.000005414267,0.00007759466],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01513762,"threshold_uncertainty_score":0.4714288,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008592765328354866,"score_gpt":0.3141079364584258,"score_spread":0.3055151711300709,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}