{"id":"W4406707721","doi":"10.1039/d4sc06819j","title":"Interplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study","year":2025,"lang":"en","type":"article","venue":"Chemical Science","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Azrieli Foundation; Israel Science Foundation; H2020 European Research Council; Weizmann Institute of Science; Deutsche Forschungsgemeinschaft; European Commission","keywords":"Förster resonance energy transfer; Single-molecule FRET; Substrate (aquarium); Chemistry; Biophysics; Enzyme; Dynamics (music); Molecule; Conformational change; Molecular dynamics; Stereochemistry; Computational chemistry; Biochemistry; Biology; Fluorescence; Physics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004807001,0.0002450466,0.000466877,0.0001598539,0.0002702729,0.0003594273,0.0006324255,0.0005634395,0.0005333882],"category_scores_gemma":[0.0003440965,0.0001771661,0.0002498359,0.0001759243,0.0005984021,0.001029031,0.0003254247,0.0009271389,0.0001647188],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003991874,"about_ca_system_score_gemma":0.0001484939,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003934417,"about_ca_topic_score_gemma":0.00027927,"domain_scores_codex":[0.9998641,0.00001987292,0.000006571651,0.00005182271,0.00003376216,0.00002391718],"domain_scores_gemma":[0.9998748,0.0000613732,0.00002185434,0.00001512285,0.00001095133,0.00001590425],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000689368,0.00008585307,0.0004402578,0.00005652316,0.00001258634,0.0001235688,0.0000797413,0.001260298,0.9903347,0.00239152,0.0001475299,0.004998497],"study_design_scores_gemma":[0.00003192412,0.0003170039,0.003911613,0.00001969644,0.00003465935,0.000537636,0.0001854894,0.05930804,0.9310508,0.001573378,0.002992252,0.00003747955],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9806538,0.002655357,0.01397617,0.0005342275,0.00003507098,0.00002301312,0.00008671819,0.00008952161,0.001946048],"genre_scores_gemma":[0.9925283,0.00140309,0.005218281,0.00008681622,0.00002101399,0.00001903996,0.00006296198,0.00001886823,0.0006415712],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006324255,"threshold_uncertainty_score":0.002896309,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008592765328354866,"score_gpt":0.3141079364584258,"score_spread":0.3055151711300709,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}