{"id":"W4406708039","doi":"10.1021/acs.bioconjchem.4c00457","title":"5′-Amino-Formyl-Thieno[3,2-<i>b</i>]thiophene End-Label for On-Strand Synthesis of Far-Red Fluorescent Molecular Rotors and pH-Responsive Probes","year":2025,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Fluorescence; Oligonucleotide; Knoevenagel condensation; Thiophene; Combinatorial chemistry; DNA; Photochemistry; Topology (electrical circuits); Molecule; Stereochemistry; Organic chemistry; Biochemistry; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001619048,0.0004026558,0.0001702945,0.0001438217,0.0001447525,0.0001511617,0.0002962052,0.0003617611,0.00195059],"category_scores_gemma":[0.0001613551,0.0001953352,0.0001596744,0.0001194033,0.0001758047,0.0002081441,0.0001767558,0.0004641493,0.0008531969],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001861663,"about_ca_system_score_gemma":0.0001848764,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002588824,"about_ca_topic_score_gemma":0.0005359113,"domain_scores_codex":[0.9998958,0.00002218639,0.000005662385,0.00002547202,0.00002739293,0.00002340761],"domain_scores_gemma":[0.9998974,0.00002679422,0.00002801369,0.00001586765,0.00001376095,0.00001825862],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001848331,0.000008910397,0.00002622558,0.00004353414,0.000001888412,0.00003443413,0.00001448453,0.00008635812,0.9983804,0.0001929892,0.00003369252,0.001158641],"study_design_scores_gemma":[0.000004723599,0.0001381664,0.0002591663,0.000003043935,0.00000330872,0.00009561503,0.000006811206,0.0004673918,0.9964405,0.0000406389,0.002536491,0.000004063195],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8823581,0.002224901,0.1062582,0.0001768108,0.0001158082,0.0001750069,0.000327032,0.0004704955,0.00789378],"genre_scores_gemma":[0.929925,0.001640497,0.05979829,0.0001126425,0.00002529552,0.0001720368,0.0004407824,0.00007327135,0.007812172],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00195059,"threshold_uncertainty_score":0.006525397,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007510665174411708,"score_gpt":0.2606543392639563,"score_spread":0.2531436740895445,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}