{"id":"W4406708039","doi":"10.1021/acs.bioconjchem.4c00457","title":"5′-Amino-Formyl-Thieno[3,2-<i>b</i>]thiophene End-Label for On-Strand Synthesis of Far-Red Fluorescent Molecular Rotors and pH-Responsive Probes","year":2025,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Fluorescence; Oligonucleotide; Knoevenagel condensation; Thiophene; Combinatorial chemistry; DNA; Photochemistry; Topology (electrical circuits); Molecule; Stereochemistry; Organic chemistry; Biochemistry; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002312952,0.0003638999,0.0004326504,0.00007959988,0.0001151179,0.00003009822,0.0002428419,0.0003637367,0.000003678016],"category_scores_gemma":[0.0003731325,0.000325018,0.0002050667,0.0001729315,0.000331347,0.000005275129,0.000122451,0.000117425,4.043046e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000314385,"about_ca_system_score_gemma":0.0001096997,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007359987,"about_ca_topic_score_gemma":0.00000239578,"domain_scores_codex":[0.9983252,0.00004025064,0.0004450776,0.0006746847,0.0001635936,0.0003511971],"domain_scores_gemma":[0.9988455,0.00006939372,0.0002107864,0.0005340702,0.0002334178,0.0001068819],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007453117,0.0001671534,0.00009756895,0.000359375,0.0002281019,0.000004246236,0.000009229793,0.000002692866,0.9906654,0.00007342209,0.0004169324,0.007230505],"study_design_scores_gemma":[0.0008732242,0.0002025924,0.00008168115,0.0002298575,0.0001474778,0.000007293409,0.000062028,0.00003455491,0.9918036,0.0002098081,0.006013156,0.0003346792],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9953604,0.001109367,0.001008293,0.000453978,0.00004758317,0.0007284732,0.000245672,0.00005421939,0.0009919555],"genre_scores_gemma":[0.9934937,0.0006164016,0.004697953,0.0003085389,0.00006107457,0.0001579401,0.0001182878,0.00003193935,0.0005141877],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006895826,"threshold_uncertainty_score":0.9999202,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007510665174411708,"score_gpt":0.2606543392639563,"score_spread":0.2531436740895445,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}