{"id":"W4406709707","doi":"10.21769/bioprotoc.5216","title":"Generation, Propagation, and Titering of Dicistrovirus From an Infectious Clone","year":2025,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"clone (Java method); Virology; Biology; Computational biology; Genetics; DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001066382,0.0009470819,0.0007172545,0.0009896383,0.0007524478,0.0006039298,0.001133495,0.0006539464,0.002246022],"category_scores_gemma":[0.0006811899,0.0006998192,0.0006957631,0.0004801693,0.000492282,0.0004969797,0.0008762084,0.001884239,0.002653385],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007952701,"about_ca_system_score_gemma":0.0007530345,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001950136,"about_ca_topic_score_gemma":0.003197923,"domain_scores_codex":[0.9994068,0.00008814754,0.00009481161,0.000127665,0.00020108,0.00008143348],"domain_scores_gemma":[0.9994769,0.0001256641,0.00005726859,0.0001671717,0.0001087945,0.00006421599],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002379293,0.00002035831,0.0001025792,0.0001019349,0.000006415223,0.00007155645,0.00004801512,0.00007788036,0.996356,0.000409496,0.0002877417,0.002494335],"study_design_scores_gemma":[0.00001613942,0.0001420865,0.0008274014,0.00001925346,0.00002859252,0.0002633033,0.00002573716,0.0009500413,0.9842551,0.0001045095,0.01334865,0.00001918231],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3487714,0.004245006,0.6075388,0.0005230707,0.0007222841,0.006587167,0.01306942,0.003488618,0.01505428],"genre_scores_gemma":[0.378541,0.004407598,0.5560897,0.0002563572,0.0001361419,0.003768742,0.03282347,0.0011184,0.02285859],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002246022,"threshold_uncertainty_score":0.007513702,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05127488123932373,"score_gpt":0.3365964611584544,"score_spread":0.2853215799191307,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}