{"id":"W4406716972","doi":"10.3389/fcimb.2025.1490948","title":"Visible and rapid detection of feline chaphamaparvovirus using multienzyme isothermal rapid amplification and lateral flow dipstick assay","year":2025,"lang":"en","type":"article","venue":"Frontiers in Cellular and Infection Microbiology","topic":"Animal Virus Infections Studies","field":"Agricultural and Biological Sciences","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Nanyang Normal University; National Natural Science Foundation of China","keywords":"Dipstick; Loop-mediated isothermal amplification; Detection limit; Polymerase chain reaction; Virology; Biology; Molecular biology; CATS; Chromatography; Chemistry; DNA; Medicine; Genetics; Gene; Biochemistry","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002914675,0.0001389347,0.0002460674,0.0001070108,0.0002411494,0.00002190517,0.00003305313,0.0001683454,0.00002046571],"category_scores_gemma":[0.000045243,0.0000782805,0.00003596863,0.0002654792,0.0002524149,0.0001040731,0.00006611165,0.0001224766,7.523952e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003510671,"about_ca_system_score_gemma":0.000004842719,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005631056,"about_ca_topic_score_gemma":0.0002409147,"domain_scores_codex":[0.9990856,0.0001603561,0.0002611349,0.0002952424,0.0000227771,0.0001749011],"domain_scores_gemma":[0.9997185,0.00006595068,0.00009748111,0.00003914078,0.00005167467,0.00002721939],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006211,0.00004578789,0.09118389,0.00002089244,0.00002739494,2.521016e-7,0.00006607555,0.00001484968,0.8521193,0.00002195215,0.00004891815,0.05638858],"study_design_scores_gemma":[0.002171622,0.001222573,0.4625527,0.0001253418,0.0001651628,0.00007319023,0.0005348194,0.02491776,0.4713027,0.0007273966,0.03548669,0.0007200539],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9858808,0.00317317,0.0100803,0.00005481266,0.0004799597,0.0002081829,0.00002413068,0.00002637545,0.00007226143],"genre_scores_gemma":[0.997726,0.001416217,0.0006671441,0.00005569934,0.00005616058,0.000009114913,0.00002313094,0.000001419481,0.00004508798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3808166,"threshold_uncertainty_score":0.3192186,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01286794970907133,"score_gpt":0.216242455762852,"score_spread":0.2033745060537807,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}