{"id":"W4406716972","doi":"10.3389/fcimb.2025.1490948","title":"Visible and rapid detection of feline chaphamaparvovirus using multienzyme isothermal rapid amplification and lateral flow dipstick assay","year":2025,"lang":"en","type":"article","venue":"Frontiers in Cellular and Infection Microbiology","topic":"Animal Virus Infections Studies","field":"Agricultural and Biological Sciences","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Nanyang Normal University; National Natural Science Foundation of China","keywords":"Dipstick; Loop-mediated isothermal amplification; Detection limit; Polymerase chain reaction; Virology; Biology; Molecular biology; CATS; Chromatography; Chemistry; DNA; Medicine; Genetics; Gene; Biochemistry","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001117827,0.0006482463,0.0005342863,0.001327048,0.0002384596,0.0005038127,0.0006411172,0.0007964468,0.0004966886],"category_scores_gemma":[0.001502401,0.0003490955,0.0003781024,0.0004476872,0.0005368973,0.0005404315,0.0005695205,0.0006668828,0.0003417302],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000475422,"about_ca_system_score_gemma":0.0004065741,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00134846,"about_ca_topic_score_gemma":0.002334827,"domain_scores_codex":[0.9977876,0.000404368,0.0001260684,0.0005761007,0.0009093977,0.0001965246],"domain_scores_gemma":[0.9992763,0.0002608621,0.0001792004,0.00005391832,0.0001690208,0.00006072302],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001433496,0.00007042253,0.007741372,0.0002214584,0.00002200817,0.0001167486,0.0001005918,0.000186663,0.9733987,0.0001447917,0.000093756,0.01776009],"study_design_scores_gemma":[0.00002857399,0.0007903451,0.05078176,0.00006177746,0.00009818661,0.002014273,0.0001776728,0.01150869,0.9300823,0.0002134863,0.004148878,0.00009398976],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8315001,0.00640537,0.1550762,0.0002088081,0.0001494559,0.0004884699,0.00100906,0.0009258468,0.004236658],"genre_scores_gemma":[0.8488441,0.001148888,0.1471344,0.0001615435,0.00003920142,0.0002977914,0.0008320305,0.0000197204,0.001522362],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00134846,"threshold_uncertainty_score":0.005911708,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01286794970907133,"score_gpt":0.216242455762852,"score_spread":0.2033745060537807,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}