{"id":"W4406746033","doi":"10.1186/s12896-025-00945-x","title":"Optimization of chemical transfection in airway epithelial cell lines","year":2025,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"St. Paul's Hospital; University of British Columbia","funders":"Institute of Circulatory and Respiratory Health; Canadian Institutes of Health Research; Michael Smith Health Research BC; Canadian Allergy, Asthma and Immunology Foundation","keywords":"Transfection; Viability assay; Lipofectamine; Biology; Molecular biology; Cell culture; Respiratory epithelium; Epithelium; Gene; Recombinant DNA; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005623268,0.00007848149,0.0001126003,0.0001395715,0.00000961779,0.000002429165,0.0001341969,0.0004743465,0.000009742619],"category_scores_gemma":[0.00003294414,0.00007858939,0.00005101616,0.0001804951,0.00008227322,0.000001758163,0.00004312957,0.00008477485,0.000002600565],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008441272,"about_ca_system_score_gemma":0.00004888972,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002568515,"about_ca_topic_score_gemma":0.0000506395,"domain_scores_codex":[0.9994529,0.00002004291,0.0001807236,0.0002066817,0.00002853769,0.0001111056],"domain_scores_gemma":[0.9997351,0.000006109133,0.00003307615,0.0001817905,0.00003493437,0.000008975792],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009649618,0.00006862291,0.0008927529,0.00002799935,0.000006310887,2.914639e-7,0.000005812932,0.002393986,0.9945998,0.00009464751,0.00009776796,0.001715569],"study_design_scores_gemma":[0.0004420923,0.0001416671,0.0001101081,0.00001512943,0.000006934139,0.000001626302,0.00002816106,0.001332537,0.9971198,0.0000592053,0.0006751596,0.00006750997],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8788275,0.0003119671,0.119958,0.0002567568,0.0001209139,0.00009598966,0.000006378415,0.00001561122,0.0004069963],"genre_scores_gemma":[0.9948222,0.0003708787,0.004498837,0.00005194598,0.00002949468,0.000012241,0.00003247449,0.000005446095,0.0001764789],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1159948,"threshold_uncertainty_score":0.3658595,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005871999439405883,"score_gpt":0.2275067036634369,"score_spread":0.2216347042240311,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}