{"id":"W4406862525","doi":"10.1042/bcj20240747","title":"Structural and kinetic characterization of an acetoacetyl-Coenzyme A: acetate Coenzyme A transferase from the extreme thermophile <i>Thermosipho melanesiensis</i>","year":2025,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Novelis (Canada)","funders":"National Institute of General Medical Sciences; National Institute of Food and Agriculture; National Institutes of Health; U.S. Department of Agriculture; U.S. Department of Energy","keywords":"Cofactor; Enzyme; Transferase; Coenzyme A; Stereochemistry; Chemistry; Biochemistry; Non-competitive inhibition; Binding site; Alanine; Acetyl-CoA; Product inhibition; Amino acid","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008887008,0.0001898577,0.0001498881,0.00009048612,0.00007461213,0.0001757776,0.0001693166,0.0001510739,0.0003265787],"category_scores_gemma":[0.0001594652,0.00009097695,0.0002489587,0.0001649405,0.0001055672,0.0001169809,0.00008398371,0.0002615571,0.0001474431],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003076633,"about_ca_system_score_gemma":0.0001717249,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003411835,"about_ca_topic_score_gemma":0.0019738,"domain_scores_codex":[0.9999524,0.000005388551,0.000004219233,0.00001185408,0.00001743456,0.000008743354],"domain_scores_gemma":[0.9999036,0.00001370571,0.00003401305,0.000009059449,0.00002156764,0.00001811853],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001441385,0.00002802486,0.001749385,0.00003230952,0.000009164409,0.00005153243,0.00001951047,0.0003222925,0.9959337,0.00005297046,0.00001844753,0.001638512],"study_design_scores_gemma":[0.00001237431,0.0002377503,0.02306852,0.000005360291,0.00002279805,0.0003952548,0.00004219168,0.004861986,0.9695332,0.00003254607,0.001776976,0.00001101547],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984126,0.000244617,0.0008954069,0.00001967791,0.000002019999,0.000005204828,0.0001893657,0.00001139182,0.0002197254],"genre_scores_gemma":[0.9966089,0.0002058546,0.001510179,0.000008046285,0.000001745884,0.000007049226,0.001007345,0.00001016922,0.00064085],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003411835,"threshold_uncertainty_score":0.006783962,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007798177631730649,"score_gpt":0.2087546714819422,"score_spread":0.2009564938502116,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}