{"id":"W4406902722","doi":"10.1039/d4an01212g","title":"Enzyme-free temperature resilient amplification assay with toehold stem-loop probe","year":2025,"lang":"en","type":"article","venue":"The Analyst","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Armand Frappier Museum; Dawson College; Institut National de la Recherche Scientifique","funders":"Mitacs","keywords":"Loop (graph theory); Chemistry; Biophysics; Biology; Mathematics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001168587,0.0009364862,0.0005190804,0.0005136624,0.0002464334,0.0004814462,0.0009018677,0.001051854,0.00121718],"category_scores_gemma":[0.001231162,0.0005409193,0.0004550567,0.0003687974,0.000511035,0.0005904611,0.000523573,0.00141284,0.001211231],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004368996,"about_ca_system_score_gemma":0.0003810227,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003000729,"about_ca_topic_score_gemma":0.0007318726,"domain_scores_codex":[0.9977731,0.0004245534,0.0001376369,0.0006575104,0.0008539342,0.0001533014],"domain_scores_gemma":[0.9991452,0.0003368294,0.0001728358,0.00009724135,0.0001874238,0.00006048907],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001779664,0.00001992747,0.00004809204,0.00005312785,0.000004130313,0.00001685384,0.00002581394,0.0001712334,0.9972691,0.0001118632,0.00003848053,0.002223549],"study_design_scores_gemma":[0.000003229859,0.0001083199,0.0001223343,0.000003573014,0.000006173379,0.00006475266,0.00000861971,0.002182696,0.9964548,0.00004895443,0.0009857641,0.00001077417],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2295098,0.001832345,0.7621254,0.0002080799,0.0001267841,0.0004495795,0.000440538,0.002099714,0.003207685],"genre_scores_gemma":[0.5430145,0.001638857,0.4442937,0.0002148368,0.00003502836,0.0005778071,0.001192199,0.0001638616,0.008869113],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00121718,"threshold_uncertainty_score":0.006180167,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007145601903108341,"score_gpt":0.2485629076631653,"score_spread":0.2414173057600569,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}