{"id":"W4407083815","doi":"10.1021/acssynbio.4c00569","title":"Characterization of Rationally Designed CRISPR/Cas9-Based DNA Methyltransferases with Distinct Methyltransferase and Gene Silencing Activities in Human Cell Lines and Primary Human T Cells","year":2025,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre for Global Health Research","funders":"National Institute of Biomedical Imaging and Bioengineering; National Institute of Allergy and Infectious Diseases; National Institute on Aging; Consejo Nacional de Ciencia y Tecnología; American Association of Immunologists; National Science Foundation Graduate Research Fellowship Program; National Institute of General Medical Sciences; Dunn Foundation; American Heart Association; Fulbright Program","keywords":"Biology; DNA methylation; Methyltransferase; Epigenetics; DNA methyltransferase; Methylation; Gene silencing; Genetics; Epigenome; Epigenomics; Computational biology; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001803325,0.000193955,0.0002771986,0.0001395062,0.00007891689,0.00001299411,0.00008539791,0.0001539252,0.000005533347],"category_scores_gemma":[0.0000158006,0.0001752148,0.0000308095,0.00009006018,0.000199049,0.000005751223,0.00002886201,0.00007472414,5.120772e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000938735,"about_ca_system_score_gemma":0.00006357038,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004199976,"about_ca_topic_score_gemma":0.00008887872,"domain_scores_codex":[0.9990324,0.0001159393,0.0002695133,0.0003534827,0.00005011681,0.0001785624],"domain_scores_gemma":[0.9996161,0.00007798276,0.00004887185,0.000177065,0.00004360703,0.00003632082],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008021448,0.00007215542,0.003243185,0.0001848867,0.00002523408,0.000001544093,0.00008284982,0.00009049269,0.9954626,0.00007187091,0.000001270512,0.0006836994],"study_design_scores_gemma":[0.0007057257,0.0003712783,0.0127059,0.00005406496,0.00005306856,0.000002912919,0.00003874065,0.0000327764,0.9857668,0.00003726008,0.0000545213,0.0001769565],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9155106,0.0005136182,0.08352957,0.00006464659,0.00003304017,0.0002228594,0.00004891652,0.000009828476,0.00006691072],"genre_scores_gemma":[0.9985355,0.0001541889,0.0008637822,0.00009556019,0.00002577098,0.00003673026,0.0002302974,0.0000170643,0.00004107345],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08302493,"threshold_uncertainty_score":0.7145051,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00598384813080284,"score_gpt":0.2676104696603606,"score_spread":0.2616266215295578,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}