{"id":"W4407083815","doi":"10.1021/acssynbio.4c00569","title":"Characterization of Rationally Designed CRISPR/Cas9-Based DNA Methyltransferases with Distinct Methyltransferase and Gene Silencing Activities in Human Cell Lines and Primary Human T Cells","year":2025,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre for Global Health Research","funders":"National Institute of Biomedical Imaging and Bioengineering; National Institute of Allergy and Infectious Diseases; National Institute on Aging; Consejo Nacional de Ciencia y Tecnología; American Association of Immunologists; National Science Foundation Graduate Research Fellowship Program; National Institute of General Medical Sciences; Dunn Foundation; American Heart Association; Fulbright Program","keywords":"Biology; DNA methylation; Methyltransferase; Epigenetics; DNA methyltransferase; Methylation; Gene silencing; Genetics; Epigenome; Epigenomics; Computational biology; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004299187,0.0003528165,0.0003378599,0.0001693143,0.0001234043,0.0004526484,0.000437383,0.0003820977,0.0007949902],"category_scores_gemma":[0.0003921259,0.000193517,0.0003645706,0.0002391523,0.0001861883,0.0001176823,0.0001384072,0.0005203818,0.0006032329],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000613355,"about_ca_system_score_gemma":0.0004063406,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00171313,"about_ca_topic_score_gemma":0.002349839,"domain_scores_codex":[0.9997202,0.00004210646,0.0000468093,0.0000688209,0.00009145155,0.00003061774],"domain_scores_gemma":[0.9998622,0.00002569904,0.00003625096,0.00001877739,0.00003272235,0.00002435278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006227838,0.00002949318,0.0001727066,0.00003847769,0.00001210573,0.00004288471,0.00001214024,0.000731505,0.9972982,0.0001253874,0.00009399794,0.001380847],"study_design_scores_gemma":[0.00001339222,0.0001382619,0.0002739523,0.000002417978,0.00001151293,0.00007385079,0.000006069226,0.001096118,0.9964303,0.00001851685,0.001931862,0.000003759494],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9478879,0.002977032,0.04151557,0.0002993908,0.00007335068,0.0003583691,0.003226693,0.0004325427,0.003229117],"genre_scores_gemma":[0.9613297,0.001580644,0.03032485,0.000131571,0.00001187912,0.0001567597,0.003388365,0.0001151117,0.002961178],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00171313,"threshold_uncertainty_score":0.004450262,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00598384813080284,"score_gpt":0.2676104696603606,"score_spread":0.2616266215295578,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}