{"id":"W4407170893","doi":"10.1101/2025.02.05.636639","title":"Heterologous production, purification and crystallization of 24C-sterol methyltransferase from <i>Candida albicans</i>","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Steroid Chemistry and Biochemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"","keywords":"Candida albicans; Heterologous; Sterol; Microbiology; Chemistry; Production (economics); Crystallization; Biology; Biochemistry; Cholesterol; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002272995,0.0004412113,0.0004101849,0.00006789449,0.00008726023,0.00005863358,0.0003656328,0.0008589576,0.00001445942],"category_scores_gemma":[0.0001757477,0.0004923776,0.0001188462,0.0001610671,0.0001651781,0.000009028357,0.0002527037,0.0003363043,8.357636e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004070795,"about_ca_system_score_gemma":0.0002705601,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004246834,"about_ca_topic_score_gemma":0.000003487351,"domain_scores_codex":[0.9977614,0.00009460991,0.0005264555,0.001142624,0.0002070164,0.0002678991],"domain_scores_gemma":[0.997953,0.00001827003,0.0003301703,0.001151867,0.0004146863,0.0001320302],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001290592,0.0001082675,0.003237328,0.0005497966,0.0002207983,0.000002231993,0.000007315689,0.0000602793,0.9953951,0.000004353441,0.0002808074,0.000004615241],"study_design_scores_gemma":[0.000404458,0.00004950434,0.005180676,0.0002043091,0.0001666328,3.883343e-8,0.000003660157,0.00002646229,0.9909688,0.000002644548,0.002590881,0.0004019773],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9913493,0.002488724,0.004081887,0.0001887321,0.0004659162,0.0004256873,0.0009297133,0.00005750011,0.00001252546],"genre_scores_gemma":[0.9965125,0.001042971,0.001541613,0.0001556385,0.0005063177,0.0001302879,0.00003381685,0.00004108972,0.00003572752],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005163223,"threshold_uncertainty_score":0.9997528,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006900760787470377,"score_gpt":0.2066792387602647,"score_spread":0.1997784779727943,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}