{"id":"W4407170893","doi":"10.1101/2025.02.05.636639","title":"Heterologous production, purification and crystallization of 24C-sterol methyltransferase from <i>Candida albicans</i>","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Steroid Chemistry and Biochemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"","keywords":"Candida albicans; Heterologous; Sterol; Microbiology; Chemistry; Production (economics); Crystallization; Biology; Biochemistry; Cholesterol; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002524783,0.0004979126,0.0003222764,0.0002116378,0.0002619282,0.0003872732,0.0005128752,0.0002443015,0.0005595827],"category_scores_gemma":[0.0002392423,0.0001317263,0.0003743598,0.0005786243,0.0001990542,0.0001416431,0.0002212189,0.000534803,0.0004057213],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007420753,"about_ca_system_score_gemma":0.0005524908,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004341638,"about_ca_topic_score_gemma":0.003316444,"domain_scores_codex":[0.9998209,0.00002713385,0.0000285106,0.00004081174,0.00005083557,0.00003179189],"domain_scores_gemma":[0.9999293,0.000008839795,0.00001579856,0.0000153621,0.00001754628,0.00001307539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009135214,0.00003137623,0.0003365488,0.00008326667,0.00001182735,0.00008070099,0.00002319105,0.0003456332,0.9966944,0.0002405037,0.0001950186,0.001866081],"study_design_scores_gemma":[0.00001618579,0.0000793225,0.001806179,0.000009248104,0.00002096775,0.0001973425,0.000028571,0.001691439,0.9890391,0.00006557778,0.007035691,0.00001046179],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9693547,0.0034611,0.01815685,0.0003062149,0.0001240851,0.0001801151,0.004548571,0.0003681861,0.003500125],"genre_scores_gemma":[0.9410918,0.002349906,0.03611207,0.00009839838,0.00002926673,0.0001348732,0.01482395,0.0001235884,0.005236196],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004341638,"threshold_uncertainty_score":0.00863272,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006900760787470377,"score_gpt":0.2066792387602647,"score_spread":0.1997784779727943,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}